A simple and rapid screening method for grain zinc content in pearl millet

Velu, G and Bhattacharjee, R and Rai, K N and Sahrawat, K L and Longvah, T (2008) A simple and rapid screening method for grain zinc content in pearl millet. Journal of SAT Agricultural Research, 6. pp. 1-4. ISSN 0973-3094

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Field and laboratory studies were conducted in Andhra Pradesh, India, to evaluate the effectiveness of a rapid screening method using Perl's Prussian blue stain for screening grain samples from different pearl millet genotypes for zinc (Zn) content. A total of 29 entries representing a wide range of grain Zn concentrations was evaluated in 5 different seasons for genotype × environment interaction. The estimation of grain Zn content was done using the sib-mated grain samples from the 2005 rainy and 2006 summer seasons following the AAS method. Of these, 9 pearl millet genotypes with a wide range of grain Zn content were chosen to standardize the DTZ staining procedure. The procedure was further validated using the grain samples of the remaining 20 entries obtained from sib-mating. Results showed that whole grain flour of pearl millet genotypes with different Zn concentrations, when treated with DTZ stain, showed an increase in the intensity of red colour formation with increasing Zn concentration. The intensity of red colour varied in genotypes having low, medium and high grain Zn content. In genotypes having high Zn content, the red colour was more intense than in those having medium Zn content. The intensity of red colour was very less or none in genotypes having less than 37 mg Zn/kg. DTZ staining can therefore be useful at least in separating flour samples with Zn concentrations lower than 40 mg/kg. These results suggested that DTZ staining was effective in separating genotypes with high and low Zn contents. The cost analysis of DTZ staining protocol showed that the chemical cost per sample was approximately US$ 0.75, which showed that this method was simple, rapid and inexpensive.

Item Type: Article
Subjects: Mandate crops > Millets
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 15 Oct 2011 08:12
Last Modified: 15 Oct 2011 08:12
URI: http://oar.icrisat.org/id/eprint/2605
Official URL:
Funders: Consultative Group on International Agricultural Research-HarvestPLus Challenge Program
Acknowledgement: The authors are grateful to HarvestPlus Challenge Program of the Consultative Group on International Agricultural Research (CGIAR) for the financial support.
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