Molecular characterization of cytoplasmicnuclear Male sterillty (cms) sources and Tallidwarf near-isocenic lines in pearl millet

Ashok Kumar, C (1995) Molecular characterization of cytoplasmicnuclear Male sterillty (cms) sources and Tallidwarf near-isocenic lines in pearl millet. PhD thesis, College of Agriculture Hisar Haryana.

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Supervisors

Supervisors NameSupervisors ID
Rai, K NICRISAT
Hash, C TICRISAT
Khairwal, I SCCS HAU

Abstract

The conventional method of classifying CMS lines on fertility restoration atterns is cumbersome and time consuming. Restriction fragment length polymorphism (RFL P ) of mitochondria1 (mt) DNA provides a rapid and effective method to assess heterogeneity among male-sterile cytoplasms. With this objective, six isonuclear A-lines (81A, with Tift 23A, cytoplasm, ICMA 88001 (= 81 A,) with violaceum cytoplasm, 81 A (= 81 A ) with monodii = violaceum cytoplasm, Pb 310A, and Pb 31 1A, with A, cytoplasm, a n $ ~ b4 06a with A cytoplasm), nine male-sterile lines from Large-seeded Genepool ( LSGP 6, LSGP 14, ~ G17P, LS GP 22, LSGP 28, LSGP 36, LSGP 43, LSGP 55 and LSGP 66) and two CMS lines each from Early Genepool (EGP 1 and EGP 2) and Population Varieties (PV 1 and PV 2) were characterized for variation in their mitochondrial genomes following Southern blot hybridizations using homologous (pearl millet 13.6 kb, 10.9 kb, 9.7 kb and 4.7 kb clones) and heterologous (maize atp6 and cox1 clones) mtDNA probes. Based on RFLP banding pattern we identified seven cytoplasmic groups from the LSGP. Of the LSGP cytoplasms, LSGP 43, LSGP 55 and LSGP 66 were most diverse......

Item Type: Thesis (PhD)
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Mandate crops > Millets
Depositing User: Mr T David
Date Deposited: 15 Oct 2011 09:24
Last Modified: 18 Nov 2011 06:00
URI: http://oar.icrisat.org/id/eprint/2601
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