Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum

    Girijashankar, V and Sharma, K K and Balakrishna, P and Seetharama, N (2007) Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum. Journal of SAT Agricultural Research, 3 (1). pp. 1-3.

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    Abstract

    Sorghum (Sorghum bicolor) is an important staple food crop, especially of the poor in Africa, Asia and Central America and needs to be improved through genetic engineering. Generation of transgenic plants depends mainly on the standardization of an efficient tissue culture and regeneration protocol. Sorghum tissue culture and regeneration protocols mostly follow either organogenesis (Maheswari et al. 2006) or direct somatic embryogenesis pathway (Harshavardhan et al. 2002). Following the protocol of Girijashankar et al. (2005), we attempted to produce transgenic sorghum with synthetic Bt gene constructs (ubicry1Ab and ubicry1Ac, separately). However, molecular analysis of the T0 and their progeny plants revealed the loss of germline transmission of the respective transgenes under study. Recent studies by Wang et al. (2005) with Agrobacterium-mediated transformation of maize (Zea mays) and regeneration through embryogenic callus, reported the absence of transgenes in the progeny plants. We report here the occurrence of different types of calli, few of which are not expected to occur during the direct somatic embryogenesis pathway of sorghum regeneration protocol followed to obtain the above transgenic plants.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Sorghum
    Depositing User: Library ICRISAT
    Date Deposited: 27 Oct 2011 05:23
    Last Modified: 27 Oct 2011 05:23
    URI: http://oar.icrisat.org/id/eprint/2574
    Official URL:
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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