Shireen Meher, K
(2003)
Optimization of Helicoverpa annigera NPV inoivo mass production and regulation of malodor associated with the process.
Masters thesis, Jawaharlal Nehru Technological University.
Supervisors
| Supervisors Name | Supervisors ID |
|---|
| Rao, G V R | ICRISAT |
| Amjaneyulu, Y | JNTU |
Abstract
The indigenous production of Heltcoverpn nrnttgcrn nuclear polyhedrosis virus
(NPV) has been proficiently employed by several farmers after realising its
prominence as a prospective biopesticide. The only issue of concern outlined by
them being the malodor associated with the recovery process, which the other
farmers in the community often complained with. Also the recovery of the virus
needed to be quantitatively optimized to enhance its efficiency and cconomy as a
microbial biopesticide.
An endeavor has been made in this regard to quantify the viral recovery at
diffcrent post inoculation (PI) days to obtain the maximum poly inclusion bodies
(PIBs) and to regulate the malodor through several techniques. These studies
were conducted at International Crops Research Institute for the Semi-Arid
Tropics, Patancheru, Andhra Pradesh.
Maximum larval mortality was found to be 88% on 7" day of PI followed by 50%
on 6" day of PI. The NPV yield was maximum, 0.70 LE/larva at 71h day followed
by 0.64 LE/Iarva at 6b day of PI. The ideal period of viral harvest can be
suggested to be 6" day of PI when the mortality percent and NPV yield were in
accord for optimal viral recovery to avoid the constraint of malodor associated
with the Helicmrpa annigera NPV production.
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