Production and characterization of monoclonal antibodies for aflatoxin B1

Devi, K T and Mayo, M A and Reddy, K L N and Delfosse, P and Reddy, G and Reddy, S V and Reddy, D V R (1999) Production and characterization of monoclonal antibodies for aflatoxin B1. Letters In Applied Microbiology, 29 (5). pp. 284-288.

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Hybridomas that secreted antibodies for aflatoxin B1 were selected using two immunization protocols referred to as A and B. Protocol A is a standard immunization method and resulted in the selection of only two clones that produced monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization schedule which resulted in the generation of 10 hybridomas is described. Of the 10, one antibody was highly specific to B1, four antibodies reacted equally strongly with B1, G1 and weakly with B2. Another four reacted strongly with B1 and weakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2. Interestingly all the 10 antibodies showed little or no cross-reaction with G2.

Item Type: Article
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Users 6 not found.
Date Deposited: 10 Oct 2011 06:11
Last Modified: 10 Oct 2011 06:12
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Funders: Department for International Development(DFID)
Acknowledgement: The financial support of the UK Department For International Development ([DFID] project number R7083) is gratefully acknowledged. We are grateful to Dr Anjali Karande, Department of Biochemistry, Indian Institute of Sciences, Bangalore, for her advice in the production of monoclonal antibodies. M. Mayo is supported by the Scottish Office Agriculture, Environment and Fisheries Department, UK. This paper was submitted as journal article number 2323 by ICRISAT.
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