Field evaluation of transgenic pigeonpea plants for resistance to Helicoverpa armigera.

Gopalaswamy, S V S and Sharma, H C and Subbaratnam, G V and Sharma, K K (2008) Field evaluation of transgenic pigeonpea plants for resistance to Helicoverpa armigera. Indian Journal of Plant Protection, 36 (2). pp. 228-234. ISSN 0253-4355

PDF - Published Version Restricted to ICRISAT users only Download (480kB) | Request a copy

Abstract

Transgenic pigeon pea lines ICPL 88039 and ICPL 87 carrying the Bacillus thuringiensis cry1Ab and soyabean trypsin inhibitor (SBTI) genes were evaluated for resistance to H. armigera under field conditions in Pattancheru, Andhra Pradesh, India, during the rainy season of 2003-05, as well as under in vitro conditions using leaves, inflorescences and pods. The lack of a significant reduction in leaf feeding, larval survival, and larval weight indicated that the levels of Cry1Ab endotoxin or SBTI present in the transgenic pigeon pea plants were not sufficient. Some plants, however, showed lower levels of leaf damage, larval survival and larval weight. Due to the inadequate levels of expression of the transgenes, resistance levels were not consistent. Neonate larvae supplemented with leaf or pod under laboratory conditions can be used effectively to evaluate transgenic pigeon pea for resistance to H. armigera.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Transgenic pigeonpea,H. armigera, Field evaluation, Bacillus thuringiensis.
Subjects:	Mandate crops > Pigeonpea
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	08 Oct 2011 12:15
Last Modified:	08 Oct 2011 12:15
URI:	http://oar.icrisat.org/id/eprint/2093
Official URL:
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

View Statistics

Actions (login required)

View Item