A comparative assessment of the utility of PCR-based marker systems in pearl millet

Thudi, M and Senthilvel, S and Bottley, A and Hash, C T and Reddy, A R and Feltus, A F and Paterson, A H and Hoisington, D A and Varshney, R K (2010) A comparative assessment of the utility of PCR-based marker systems in pearl millet. Euphytica, 174 (2). pp. 253-260. ISSN 0014-2336

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A set of 22 pearl millet inbred lines including the parents of eleven mapping populations, was screened with 627 markers including 100 pearl millet genomic SSRs (gSSRs), 60 pearl millet ESTSSRs (eSSRs), 410 intron sequence haplotypes (ISHs), and 57 exon sequence haplotypes (ESHs). In all, 267 (59%) of themarkers were informative for at least one of the 11 mapping populations, which segregate for traits like drought and salinity tolerance; host plant resistance to downymildew, rust and blast; fertility restoration and sterility and maintenance of cytoplasmic male sterility etc. An average of 116 polymorphic markers was identified per mapping population. The average PIC values and number of profiles (P) per polymorphic marker were: gSSRs (PIC = 0.62, P = 6.1), ISHs (PIC = 0.39, P = 2.6), eSSRs (PIC = 0.36, P = 3.1) and ESHs (PIC = 0.35, P = 3.1). A high correlation (r[0.97, P\0.05) was observed between the patterns of diversity exposed by the different marker systems. The polymorphic markers identified are suitable for the de novo construction, or the supplementation of pearl millet linkagemaps. The genetic relationships identified among the panel of inbred lines may be useful in designing strategies to improve the use of available genetic variation in the context of pearl millet breeding.

Item Type: Article
Agro Tags: <b>Agrotags</b> - genetics | millets | pearl millet (plant) | polymorphism | dna | information needs | planting | genes | genomes | crops <br><b>Fishtags</b> - pearls <br><b>Geopoliticaltags</b> - usa | india | mexico
Subjects: Mandate crops > Millets
Depositing User: Library ICRISAT
Date Deposited: 16 Aug 2011 09:28
Last Modified: 08 Jun 2012 08:10
URI: http://oar.icrisat.org/id/eprint/205
Official URL: http://dx.doi.org/10.1007/s10681-010-0148-5
Funders: Syngenta Foundation for the Sustainable Agriculture, Government of India - Department of Biotechnology, Council of Scientific and Industrial Research(India)
Acknowledgement: Authors are thankful to the The Syngenta Foundation for the Sustainable Agriculture (SFSA, www. syngentafoundation.org/), and Department of Biotechnology of the Government of India (DBT, http://dbtindia.nic.in/) for financial support.MTis also thankful to the Council of Scientific and Industrial Research (CSIR) of the Government of India for sponsoring his fellowship.
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