Screening techniques and resistance sources for foliar blast in pearl millet

Thakur, R P and Sharma, R and Rai, K N and Gupta, S K and Rao, V P (2009) Screening techniques and resistance sources for foliar blast in pearl millet. Journal of SAT Agricultural Research, 7. pp. 1-5. ISSN 0973-3094

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Abstract

Once considered a minor disease of pearl millet, incidence of blast disease caused by Pyricularia grisea, has increased at an alarming rate in the recent past, particularly on commercial hybrids in several states of India. The disease can be best managed through host plant resistance. In this study, attempts were made to develop the field and greenhouse screening techniques and screen some elite hybrid parental lines to identify resistance to this disease. The field screening technique involved the use of a highly susceptible line as an infector row grown after every four test rows, artificial spray inoculation of 30-day-old plants using P. grisea spore suspension (1 × 105 spores ml-1) and maintaining high humidity (>90% RH) through perfo-irrigation for 2 weeks following inoculation. The greenhouse screening technique involved spray inoculation of 15-day-old potted seedlings with P. grisea spore suspension and maintaining moderate temperature (25±1°C) and high humidity through a misting system for 10 days after inoculation. In all, 211 elite hybrid parental lines, including 126 designated B-lines, 20 designated R-lines and 65 potential R-lines were evaluated for blast resistance in the disease nursery. Forty-five lines identified as blast resistant (score ≤3.0 on 1–9 scale) were further screened through greenhouse screening technique. Twenty-five (8 designated B-lines, 3 designated R-lines and 14 potential R-lines) of the 45 lines were found resistant to blast under greenhouse. All of these 25 foliar blast resistant lines were also resistant to downy mildew under field conditions. These resistant lines would be useful in breeding blast resistant pearl millet hybrids.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Mandate crops > Millets
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 07 Oct 2011 03:32
Last Modified: 17 Apr 2015 05:17
URI: http://oar.icrisat.org/id/eprint/2021
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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