Leaf chlorophyll concentration relates to transpiration efficiency in peanut

Sheshshayee, M S and Bindumadhava, H and Rachaputi, N R and Prasad, T G and Udayakumar, M and Wright, G C and Nigam, S N (2006) Leaf chlorophyll concentration relates to transpiration efficiency in peanut. Annals of Applied Biology, 148 (1). pp. 7-15. ISSN 0003-4746

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Two pot experiments were conducted in two different seasons at the University of Agricultural Science, Bangalore, India, to study (a) the relationship between chlorophyll concentration (by measuring the leaf light-transmittance characteristics using a SPAD metre) and transpiration efficiency (TE) and (b) the effect of leaf N on chlorophyll and TE relationship in peanut. In Experiment (Expt) I, six peanut genotypes with wide genetic variation for the specific leaf area (SLA) were used. In Expt II, three non-nodulating isogenic lines were used to study the effect of N levels on leaf chlorophyll concentration–TE relationship without potential confounding effects in biological nitrogen fixation. Leaf N was manipulated by applying N fertiliser in Expt II. Chlorophyll concentration, TE (g dry matter kg−1 of H2O transpired, measured using gravimetric method), specific leaf nitrogen (g N m−2, SLN), SLA (cm2 g−1), carbon isotope composition (Δ13C) were determined in the leaves sampled during the treatment period (35–55 days after sowing) in the two experiments. Results showed that the leaf chlorophyll concentration expressed as soil plant analytical development (SPAD) chlorophyll metre reading (SCMR) varied significantly among genotypes in Expt I and as a result of N application in Expt II. Changes in leaf N levels were strongly associated with changes in SCMR, TE and Δ13C. In both the experiments, a significant positive relationship between SCMR and TE with similar slopes but differing intercepts was noticed. However, correction of TE for seasonal differences in vapour pressure deficit (VPD) between the two experiments resulted in a single and stronger relationship between SCMR and TE. There was a significant inverse relationship between SCMR and Δ13C, suggesting a close linkage between chlorophyll concentration and Δ13C in peanut. This study provides the first evidence for a significant positive relationship between TE and leaf chlorophyll concentration in peanut. The study also describes the effect of growing environment on the relationships among SLA, SLN and SCMR

Item Type: Article
Subjects: Mandate crops > Groundnut
Depositing User: Users 6 not found.
Date Deposited: 05 Oct 2011 10:39
Last Modified: 05 Oct 2011 10:42
URI: http://oar.icrisat.org/id/eprint/2017
Official URL: http://dx.doi.org/10.1111/j.1744-7348.2005.00033.x
Acknowledgement: We thank Prof. Graham D. Farquhar, Group leader, Environmental Biology Group, Research School of Biological Sciences, Australian National University, Canberra, Australia, for providing the facility to determine leaf N content using the Nitrogen analyzer. The BOYSCAST fellowship awarded to M. S. S (No. HRU/BYS/L-14/99) by the Department of Science and Technology, Government of India, New Delhi, is acknowledged. Mr Nagabhushana’s and Mr Ramesha’s assistance during stable isotope analysis at Bangalore is duly acknowledged.
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