Identification of several small main-effect QTLs and a large number of epistatic QTLs for drought tolerance related traits in groundnut (Arachis hypogaea L.)

Ravi, K and Vadez, V and Isobe, S and Mir, R R and Guo, Y and Nigam, S N and Gowda, M V C and Radhakrishnan, T and Bertioli, D J and Knapp, S J and Varshney, R K (2011) Identification of several small main-effect QTLs and a large number of epistatic QTLs for drought tolerance related traits in groundnut (Arachis hypogaea L.). TAG Theoretical and Applied Genetics, 122 (6). pp. 1119-1132. ISSN 1432-2242

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Abstract

Cultivated groundnut or peanut (Arachis hypogaea L.), an allotetraploid (2n = 4x = 40), is a self pollinated and widely grown crop in the semi-arid regions of the world. Improvement of drought tolerance is an important area of research for groundnut breeding programmes. Therefore, for the identification of candidate QTLs for drought tolerance, a comprehensive and refined genetic map containing 191 SSR loci based on a single mapping population (TAG 24 9 ICGV 86031), segregating for drought and surrogate traits was developed. Genotyping data and phenotyping data collected for more than ten drought related traits in 2–3 seasons were analyzed in detail for identification of main effect QTLs (M-QTLs) and epistatic QTLs (E-QTLs) using QTL Cartographer, QTLNetwork and Genotype Matrix Mapping (GMM) programmes. A total of 105 M-QTLs with 3.48–33.36% phenotypic variation explained (PVE) were identified using QTL Cartographer, while only 65 M-QTLs with 1.3–15.01% PVE were identified using QTLNetwork. A total of 53 M-QTLs were such which were identified using both programmes. On the other hand, GMM identified 186 (8.54–44.72% PVE) and 63 (7.11–21.13% PVE), three and two loci interactions, whereas only 8 E-QTL interactions with 1.7–8.34% PVE were identified through QTLNetwork. Interestingly a number of co-localized QTLs controlling 2–9 traits were also identified. The identification of few major, many minor M-QTLs and QTL 9 QTL interactions during the present study confirmed the complex and quantitative nature of drought tolerance in groundnut. This study suggests deployment of modern approaches like marker-assisted recurrent selection or genomic selection instead of marker-assisted backcrossing approach for breeding for drought tolerance in groundnut.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Peanut, Drought tolerance, Genetic map, Molecular markers, Main-effect QTLs, Epistatic QTLs, Molecular breeding
Agro Tags: <b>Agrotags</b> - genetics | drought | locus | groundnuts | irrigation | genomes | crops | phenotypes | leaf area | tolerance <br><b>Fishtags</b> - drying <br><b>Geopoliticaltags</b> - maine | usa | india | marches | georgia | delhi | mexico | brazil
Subjects: Mandate crops > Groundnut
Depositing User: Siva Shankar
Date Deposited: 09 May 2011 09:41
Last Modified: 16 Sep 2013 09:05
URI: http://oar.icrisat.org/id/eprint/2
Official URL: http://dx.doi.org/10.1007/s00122-010-1517-0
Projects: UNSPECIFIED
Funders: Indian Council of Agricultural Research, Consultative Group for International Agricultural Research(CGIAR)
Acknowledgement: UNSPECIFIED
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