Subramanian, V and Gurtu, S and Rao, R C N and Nigam, S N (2000) Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay. Genome, 43 (4). pp. 656-660.
![[img]](http://oar.icrisat.org/style/images/fileicons/application_pdf.png)
|
PDF
Download (537kB) | Preview |
Abstract
Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement programme. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing a RAPD assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Arachis hypogaea L., RAPD, DNA polymorphism, Oligonucleotide, random primers. |
| Subjects: | Mandate crops > Groundnut |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 07 Oct 2011 05:41 |
| Last Modified: | 07 Oct 2011 05:41 |
| URI: | http://oar.icrisat.org/id/eprint/1996 |
| Official URL: | http://www.nrcresearchpress.com/doi/pdf/10.1139/g0... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric