Interaction between host plant resistance and biological activity of Bacillus thuringiensis in managing the pod borer Helicoverpa armigera in chickpea

Surekha Devi, V and Sharma, H C and Arjuna Rao, P (2011) Interaction between host plant resistance and biological activity of Bacillus thuringiensis in managing the pod borer Helicoverpa armigera in chickpea. Crop Protection, 30 (8). pp. 962-969. ISSN 0261-2194

Preview	PDF (Author version post-print) - Accepted Version Download (189kB) | Preview
	PDF Restricted to ICRISAT users only Download (325kB) | Request a copy

Abstract

The legume pod borer, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) has developed high levels of resistance to conventional insecticides, and therefore, efforts are being made to develop transgenic chickpea expressing toxin genes from the bacterium, Bacillus thuringiensis (Bt) for controlling this pest. However, there is an apprehension that acid exudates in chickpea might interfere with biological activity of Bt. Therefore, we studied the biological activity of Bt (BiolepR) on four chickpea genotypes with different levels of resistance to H. armigera under field conditions, and by incorporating lyophilized lead and pod tissue into the artificial diet with and without Bt. The pH of the acid exudates varied from 2.1 to 2.90, and malic and oxalic acid were the major components of the acid exudates in different chickpea genotypes. There was no survival of H. armigera larvae in chickpea plants spayed with 0.1, 0.2 and 0.5% of Bt. There was a significant reduction in larval survival, larval and pupal weights and fecundity, and prolongation of larval and pupal periods in chickpea plots sprayed with Bt (0.05%) as compared to the unsprayed plants. Biological activity of Bt was lower on artificial diets with leaf or pod powder of chickpea genotypes, which might be because of a low intake of Bt toxins due to antifeedant effects of acid exudates in the chickpea or reduction in biological activity of Bt due to the interaction of biochemical constituents in chickpea with the Bt toxins. Larval survival, larval and pupal weights, pupation and adult emergence were significantly lower on diets with leaf or pod powder of the H. armigera-resistant genotypes than on the susceptible check. Chickpea genotypes with resistance to H. armigera acted in concert with Bt to cause adverse effects on the survival and development of this insect. The results suggested that development of transgenic chickpeas expressing toxin genes form Bt will be quite effective for controlling of the pod borer, H. armigera.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Chickpea; Helicoverpa armigera; Bacillus thuringiensis; Host plant resistance; Acid exudates; Transgenics; Pest management
Agro Tags:	<b>Agrotags</b> - chickpeas | genotypes | rations | animal developmental stages | acidity | proteins | planting | organic acids | biological phenomena | concentrates <br><b>Fishtags</b> - drying <br><b>Geopoliticaltags</b> - india | andhra pradesh | act | delhi | usa | united kingdom | berlin
Subjects:	Mandate crops > Chickpea
Depositing User:	Siva Shankar
Date Deposited:	18 May 2011 05:20
Last Modified:	18 Mar 2014 08:05
URI:	http://oar.icrisat.org/id/eprint/19
Official URL:	http://dx.doi.org/10.1016/j.cropro.2011.03.018
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors are thankful to the staff of entomology, ICRISAT for their help in these studies.
Links:	Author

View Statistics

Actions (login required)

View Item