Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus

    Bragard, C and Duncan, G H and Wesley, S V and Naidu, R A and Mayo, M A (2000) Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus. Journal of General Virology, 81 (1). pp. 267-272. ISSN 0022-1317

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    Abstract

    cDNA copies of the coat protein (CP) gene of Indian peanut clump virus (IPCV)-H were introduced into cells of Nicotiana benthamiana or Escherichia coli by transformation with vectors based on pROKII or pET respectively. In both plant and bacterial cells, IPCV CP was expressed and assembled to form virus-like particles (VLP). In plant extracts, the smallest preponderant particle length was c. 50 nm. Other abundant lengths were c. 85 and c. 120 nm. The commonest VLP length in bacterial extracts was c. 30 nm. Many of the longer VLP appeared to comprise aggregates of shorter particles. The lengths of the supposed 'monomer' VLP corresponded approximately to those expected for encapsidated CP gene transcript RNA. Immunocapture RT-PCR, using primers designed to amplify the CP gene, confirmed that the VLP contained RNA encoding IPCV-H CP. The results show that encapsidation does not require the presence of the 5′-terminal untranslated sequence of the virus RNA and suggest that if there is an 'origin of assembly' motif or sequence, it lies within the CP gene. When transgenic plants expressing IPCV-H CP were inoculated with IPCV-L, a strain that is serologically distinct from IPCV-H, the virus particles that accumulated contained both types of CP.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Groundnut
    Depositing User: Library ICRISAT
    Date Deposited: 23 Sep 2011 09:33
    Last Modified: 23 Sep 2011 09:33
    URI: http://oar.icrisat.org/id/eprint/1891
    Official URL: http://vir.sgmjournals.org/content/81/1/267.full.p...
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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