Spatiotemporal Separation of Groundnut Rosette Disease Agents

Naidu, R A and Kimmins, F M and Holt, J and Robinson, D J and Deom, C M and Subrahmanyam, P (1999) Spatiotemporal Separation of Groundnut Rosette Disease Agents. Phytopathology, 89 (10). pp. 934-941.

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Analysis by triple-antibody sandwich enzyme-linked immunosorbent assay of groundnut samples from fields in two seasons from different regions of Malawi showed the absence of groundnut rosette assistor virus (GRAV) from some plants showing groundnut rosette disease symptoms and the presence of GRAV in some symptomless plants. Viruliferous Aphis craccivora collected from fields transmitted either GRAV alone, groundnut rosette virus (GRV) with its satellite RNA (sat RNA), or all three agents together, in different proportions. More plants became infected with all three agents when increasing numbers of potentially viruliferous aphids were used per plant, suggesting a dosage response. Electrical penetration graph studies of aphid stylet activities indicated successful transmission of GRV and its sat RNA during both the "stylet pathway phase" and salivation into sieve elements, whereas GRAV was transmitted only during the latter phase. Aphids transmitted all three agents together only during the salivation phase. Reverse-transcriptase polymerase chain reaction testing of viruliferous aphids and of inoculated plants revealed no correlation between the presence of all three agents in prospective aphid vectors and their simultaneous transmission to groundnut plants. These results show that separation of the groundnut rosette disease agents occurs over time and space.

Item Type: Article
Subjects: Mandate crops > Groundnut
Depositing User: Library ICRISAT
Date Deposited: 21 Sep 2011 10:17
Last Modified: 14 Sep 2013 09:34
Official URL:
Acknowledgement: UNSPECIFIED
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