Occurrence of ochratoxin A in black pepper, coriander, ginger and turmeric in India

Devi, K T and Mayo, M A and Reddy, G and Emmanuel, K E and Larondelle, Y and Reddy, D V R (2001) Occurrence of ochratoxin A in black pepper, coriander, ginger and turmeric in India. Food Additives and Contaminants, 18 (9). pp. 830-835.

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Ochratoxin A (OA) contamination of black pepper, coriander seeds, powdered ginger and turmeric powder was estimated using indirect competitive ELISA. Samples (1 g) were extracted with 0.5% potassium chloride (KCl) in 70% methanol (5 ml) and diluted subsequently to give two-fold to ten-fold step-wise dilutions in phosphate-buffered saline containing 0.05% Tween 20 and 0.2% bovine serum albumin (PBS-T BSA). For extracts from the spices analysed, ELISA estimates of OA concentrations were compared with those made by HPLC. All estimates were within 1-2 standard deviation of the ELISA values. More than 90% of OA added to spice samples was recovered from samples containing between 5 and 100 microg/kg OA. Extracts of OA-free spice samples contained substances that interfered with ELISA, presumably because of non-specific reactions. This effect was avoided by preparing all the test solutions in extracts of OA-free spice samples. In 126 samples obtained from retail shops, OA was found to exceed 10 microg/kg in 14 (in the range of 15-69 microg/kg) of 26 black pepper samples, 20 (in the range of 10-51 microg/kg) of 50 coriander samples, two (23 microg/kg and 80 microg/kg) of 25 ginger samples and nine (in the range of 11-102 microg/kg) of 25 turmeric samples. This is the first record in India of the occurrence of OA in what are some of the most widely used spices in Indian cooking.

Item Type: Article
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Library ICRISAT
Date Deposited: 21 Sep 2011 06:33
Last Modified: 21 Sep 2011 06:33
URI: http://oar.icrisat.org/id/eprint/1779
Official URL: http://dx.doi.org/10.1080/02652030117589
Acknowledgement: UNSPECIFIED
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