Surface sterilant effect on the regeneration efficiency from cotyledon explants of groundnut (Arachis hypogea L.) varieties adapted to eastern and Southern Africa

Maina, S M and Emongor, Q and Sharma, K K and Simon, T G and Gathaara, M and Santie, M D V (2010) Surface sterilant effect on the regeneration efficiency from cotyledon explants of groundnut (Arachis hypogea L.) varieties adapted to eastern and Southern Africa. African Journal of Biotechnology, 9 (20). pp. 2866-2871. ISSN 1684-5315

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Abstract

Five groundnut (Arachis hypogea L.) varieties - ICGV-12991, ICGV-99568, ICGV-90704, CG-2 and Chalimbana - that are adapted to Eastern and Southern Africa were compared to variety JL 24 for their regeneration response in tissue culture. Sodium hypochlorite and mercuric chloride were compared for efficiency as sterilizing agents and subsequent effect on regeneration. All five varieties formed shoot buds that elongated well on shoot elongation medium. ICGV-90704 and Chalimbana performed better than the other three varieties for shoot organogenesis although all varieties produced healthy rooted plants in vitro that were successfully transferred to the greenhouse where they exhibited normal growth, flowering and seed set. Both sterilizing agents were suitable, but mercuric chloride was less harmful than sodium hypochlorite. This study established a basis for genetic engineering activities on African groundnuts in the future.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Agro Tags:	<b>Agrotags</b> - groundnuts | varieties | planting | equipment | regeneration | biological phenomena | genetics | contamination | arachis hypogaea | leaves <br><b>Fishtags</b> - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - africa | southern africa | kenya | malawi | india | usa
Subjects:	Mandate crops > Groundnut
Depositing User:	Library ICRISAT
Date Deposited:	12 Aug 2011 09:31
Last Modified:	16 Sep 2013 03:47
URI:	http://oar.icrisat.org/id/eprint/171
Official URL:	http://www.academicjournals.org/AJB/PDF/pdf2010/17...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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