Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics

Suresh, P and Mamta, S and Krishna Kishore, G and Shivram, L and Naga Mangala, U (2010) Characterization of Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics. African Journal of Biotechnology, 9 (46). pp. 7961-7967. ISSN 1684-5315

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Abstract

Eight isolates of Botrytis cinerea, causal organism of chickpea from eight different locations of western and eastern Indo-Gangetic Plains (IGP) of India were analyzed for morphological, cultural, virulence and genetic variations. Characterization of virulence and genetic variations of the isolates was based on their pathogenicity against 40 selected chickpea genotypes and randomly amplified polymorphic DNA (RAPD) analysis, respectively. The isolates differed in their optimum growth, temperature, conidial formation and size of conidia on potato dextrose agar. Based on cluster analysis, the eight test isolates were separated into three pathotypes with two isolates from western and four from eastern IGP grouped together with >80% similarity. Based on cluster analysis of the RAPD banding patterns, genetic similarity of the isolates varied from 14-44%, and the isolates were separated into three groups. However, pathotypes variation detected among B. cinerea isolates could not be differentiated based on the RAPD markers examined.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Agro Tags:	<b>Agrotags</b> - isolation | fungi | genetics | dna | chickpeas | botrytis cinerea | planting | growth | polymorphism | rapd <br><b>Fishtags</b> - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - india | usa | nepal | netherlands | bangladesh
Subjects:	Mandate crops > Chickpea
Depositing User:	Library ICRISAT
Date Deposited:	12 Aug 2011 09:29
Last Modified:	16 Sep 2013 03:49
URI:	http://oar.icrisat.org/id/eprint/170
Official URL:	http://www.academicjournals.org/AJB/PDF/pdf2010/15...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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