Characterization of the large (L) RNA of peanut bud necrosis tospovirus

Gowda, S and Satyanarayana, T and Naidu, R A and Mushegian, A and Dawson, W O and Reddy, D V R (1998) Characterization of the large (L) RNA of peanut bud necrosis tospovirus. Archives of Virology, 143 (12). pp. 2381-2390.

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The nucleocapsids purified from groundnut plants systemically infected with peanut bud necrosis tospovirus (PBNV) contained both viral (v) and viral complementary (vc) sense L RNAs. Defective forms of L RNA containing core polymerase region were observed. The full length L RNA of PBNV was sequenced using overlapping cDNA clones. The 8911 nucleotide L RNA contains a single open reading frame (ORF) in the vc strand, and encodes a protein of 330 kDa. At the 5' and 3' termini of the v sense RNA there were 247 and 32 nt untranslated regions, respectively, containing an 18 nt complementary sequence with 1 mismatch. Comparisons of the predicted amino acid sequence of the L protein of PBNV with other members of Bunyaviridae suggest that the L protein of PBNV is a viral polymerase. The L protein had highest identity in the core-polymerase domain with the corresponding regions of tospoviruses, tomato spotted wilt tospovirus and impatiens necrotic spot tospovirus.

Item Type: Article
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Library ICRISAT
Date Deposited: 17 Sep 2011 05:39
Last Modified: 17 Sep 2011 05:39
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Acknowledgement: UNSPECIFIED
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