Identification and Characterization of Toxigenic Fusaria Associated with Sorghum Grain Mold Complex in India

Sharma, R and Thakur, R P and Senthilvel, S and Nayak, S N and Veera Reddy, S and Rao, V P and Varshney, R K (2011) Identification and Characterization of Toxigenic Fusaria Associated with Sorghum Grain Mold Complex in India. Mycopathologia, 171. pp. 223-230.

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Abstract

Fusarium species are dominant within the sorghum grain mold complex. Some species of Fusarium involved in grain mold complex produce mycotoxins, such as fumonisins. An attempt was made to identify Fusarium spp. associated with grain mold complex in major sorghum-growing areas in India through AFLP-based grouping of the isolates and to further confirm the species by sequencing part of alpha-Elongation factor gene and comparing the sequences with that available in the NCBI database. The dendrogram generated from the AFLP data clustered the isolates into 5 groups. Five species of Fusarium-F. proliferatum, F. thapsinum, F. equiseti, F. andiyazi and F. sacchari were identified based on sequence similarity of alpha-Elongation factor gene of the test isolates with those in the NCBI database. Fusarium thapsinum was identified as predominant species in Fusarium-grain mold complex in India and F. proliferatum as highly toxigenic for fumonisins production. Analysis of molecular variance (AMOVA) revealed 54% of the variation in the AFLP patterns of 63 isolates was due to the differences between Fusarium species, and 46% was due to differences between the strains within a species.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	AFLP; a-Elongation factor; Fusarium; Mycotoxin; Fumonisin
Agro Tags:	<b>Agrotags</b> - fungi | isolation | biodiversity | mycotoxins | grain | sorghum | genetic techniques | sorghum bicolor | dna | productivity <br><b>Fishtags</b> - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - india | usa | andhra pradesh
Subjects:	Mandate crops > Sorghum
Depositing User:	Siva Shankar
Date Deposited:	17 May 2011 10:24
Last Modified:	21 Nov 2017 09:18
URI:	http://oar.icrisat.org/id/eprint/14
Official URL:	http://dx.doi.org/10.1007/s11046-010-9354-x
Projects:	UNSPECIFIED
Funders:	The Sehgal Foundation Endowment Fund
Acknowledgement:	The research was supported by The Sehgal Foundation Endowment Fund instituted at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, AP, India.
Links:	Author

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