Moin, M and Vasanthrao, T and Tyagi, W (2025) Lipid signalling components identified as key targets for generating new alleles to improve grain-quality traits in pearl millet. Journal of Cereal Science (TSI), 126. ISSN 0733-5210
Full text not available from this repository. (Request a copy)Abstract
This study investigates the in silico analysis and differential expression patterns of PgDREB2, PgPDAT, and PgDGAT gene families, which are involved in lipid signalling, to understand their role in rancidity, a key grain-quality trait in pearl millet. The gene structures, protein properties, cis-regulatory elements (CREs), and expression of four PgDREB2, two PgPDAT, and two PgDGAT members were analyzed. Expression profiling of eight genes in genotypes with contrasting rancidity across key growth and developmental stages revealed that PgPDAT1 and PgDGAT1 were specifically expressed in grains. Comparative analysis of the putative promoter sequences of eight genes in the pangenome revealed multiple nucleotide variations leading to alterations in the CREs. Nucleotide polymorphism was also identified through allele mining of two grain-specific genes. However, resequencing data of the two target loci and 500 kb flanking regions across contrasting genotypes, along with Amplification Refractory Mutation System (ARMS) PCR on ten genotypes with differing rancidity profiles, indicated that these contrasting genotypes carry the same allele as the reference genome. This suggests that natural allelic variations are not linked with rancidity, underscoring PgPDAT1 and PgDGAT1 as key targets for gene editing to enhance pearl millet grain quality.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | grain-quality traits, generating new alleles, pearl millet |
| Subjects: | Others > Genetic Engineering Mandate crops > Millets > Pearl Millet |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 10 Mar 2026 03:34 |
| Last Modified: | 10 Mar 2026 03:34 |
| URI: | http://oar.icrisat.org/id/eprint/13529 |
| Official URL: | https://www.sciencedirect.com/science/article/abs/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | The authors express their profound gratitude to Dr. Shashi Kumar Gupta, the pearl millet breeder, and Mr. Pradeep Kumar, Scientific Officer in the Plant Breeding cluster at ICRISAT, for their generous provision of the seeds used in this study.... |
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