Identification of high blanchability donors, candidate genes and markers in groundnut

Shah, P and Gangurde, S S and Senthil, R and Singam, P and Peerzada, O H and Janila, P and Singh, K and Mayes, S and Pandey, M K (2025) Identification of high blanchability donors, candidate genes and markers in groundnut. BMC Plant Biology (TSI), 25. pp. 1-16. ISSN 1471-2229

PDF - Published Version Available under License ["licenses_description_cc_attribution" not defined]. Download (7MB)

Abstract

Blanchability is the ability of seeds to shed their seed coat (testa) and is a trait of economic importance in the food processing industry, yet remains underexplored in breeding programs. In this study, blanchability was evaluated in 184 groundnut accessions from the ICRISAT minicore collection to identify associated genomic regions, candidate genes, and molecular markers. Significant variability was observed over two seasons, with values ranging from 3.98 to 70.08%. Ten genotypes, including ICG10890, ICG9507, ICG13982, and ICG297, showed high blanchability, with ICG297 emerging as a promising donor based on cluster analysis of blanchability and agronomic traits. Genome-wide associations study (GWAS) using the 58 K ‘Axiom_Arachis’ SNP array revealed 58 significant SNP-trait associations, highlighting important genes such as isocitrate dehydrogenase and ubiquitin ligase, which influence seed coat structure and cell wall integrity thereby affecting blanchability. Further, nine SNPs were selected via allele mining, among these four SNPs, on chromosomes A01 (snpAH00551, AhBL01), A06 (snpAH00554, AhBL02), B04 (snpAH00558, AhBL03), and B07 (snpAH00559, AhBL04), effectively distinguishing between high and low blanchability genotypes. These validated SNPs present valuable tools for genomics-assisted breeding. Overall, the finding contributes towards better understanding of the genetic basis of blanchability in groundnut, providing key genomic resources for improving processing-related traits.

Item Type:	Article
Divisions:	Genebank Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Blanchability, Groundnut, Genome-wide association study, markers, Testa
Subjects:	Mandate crops > Groundnut Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	03 Mar 2026 11:01
Last Modified:	03 Mar 2026 11:01
URI:	http://oar.icrisat.org/id/eprint/13513
Official URL:	https://link.springer.com/article/10.1186/s12870-0...
Projects:	ICAR-ICRISAT collaborative project, Tropical Legumes III
Funders:	Indian Council of Agricultural Research, MARS Inc. USA, Bill & Melinda Gates Foundation (BMGF), USA
Acknowledgement:	Priya Shah acknowledges Joint Council of Scientific and Industrial Research-University Grant Commission (CSIR-UGC), Government of India for the award of Ph.D. fellowship and the ICRISAT Genebank for providing seed material and, to the ICRISAT for providing access to research facilities. The authors are thankful to Khaja D. Mohinuddin, for his assistance in improving manuscript figures.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item