Genome-Wide Identification and Expression Analysis of Lipoxygenase (LOX) Gene Family in Pearl Millet (Pennisetum Glaucum L.)

Palakolanu, S R and Bommineni, P R and Tyagi, W (2025) Genome-Wide Identification and Expression Analysis of Lipoxygenase (LOX) Gene Family in Pearl Millet (Pennisetum Glaucum L.). Plant Molecular Biology Reporter (TSI), 44. pp. 1-15. ISSN 0735-9640

PDF - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (3MB)

Abstract

Lipoxygenases (LOX) are non-heme, non-sulfur iron-containing enzymes involved in lipid oxidation, playing key roles in plant development and stress responses. Despite their functional significance, LOX genes remain poorly characterized in climate-resilient crops like pearl millet (Pennisetum glaucum), which is prone to grain rancidity—a major post-harvest problem that affects quality and shelf life. In this study, ten PgLOX genes were identified and classified into 9-LOX and 13-LOX subfamilies, with uneven distribution across chromosomes and evidence of tandem and segmental duplications. Evolutionary analysis indicated purifying selection. Motif analysis revealed conserved motifs within the PgLOX gene family. Cis-motif analysis showed that the potential promoter regions of the PgLOX genes contain various elements that are tissue-specific, related to development, stress-responsive, and hormone-responsive. Protein–protein interaction predictions suggested functional connections among eight PgLOX proteins. qRT-PCR analysis showed that among the ten PgLOX genes, PgLOX1 was significantly expressed in the grains of genotypes susceptible to rancidity, whereas it was not present in leaf tissues. These findings indicate that PgLOX1 may be involved in lipid peroxidation and oxidative rancidity in pearl millet grains, emphasizing its potential for functional validation and gene editing to enhance grain quality.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Gene expression, Lipoxygenases, Pearl millet, Off-flavor, Rancidity, Grain quality
Subjects:	Others > Genetic Engineering Mandate crops > Millets > Pearl Millet
Depositing User:	Mr Nagaraju T
Date Deposited:	17 Feb 2026 03:39
Last Modified:	17 Feb 2026 03:39
URI:	http://oar.icrisat.org/id/eprint/13473
Official URL:	https://link.springer.com/article/10.1007/s11105-0...
Projects:	UNSPECIFIED
Funders:	Novo Nordisk Foundation
Acknowledgement:	The authors thank Dr SK Gupta, pearl millet breeding unit, ICRISAT, India, for providing the seeds of ICMB863 and ICMB95222. Mr N Kedarinath and Mr Bramhananda Reddy are acknowledged for maintaining pearl millet plants in the glass house.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item