Abhang, S L and Sharma, P and Datarkar, S N and Moharil, M P and Kudapa, H and Ingle, A P and Jadhav, P V (2025) Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium Wilt Resistance-Related Differential Gene Expression in Chickpea. In: Plant Biotic Stress. Methods in Molecular Biology, 1 . Springer, Humana New York, NY, pp. 155-165. ISBN 978-1-0716-4745-5
Full text not available from this repository. (Request a copy)Abstract
The cDNA start codon targeted marker (cDNA-SCoT) methodology is a unique, simple, quick, and efficient method for investigating differential gene expression. The differentially expressed fragments (DEFs) in resistant and susceptible cultivars were identified using cDNA-SCoT profiling. Sanger sequencing and the nBLAST homology search program were used to characterize the functional properties of DEFs. As a result, this methodology is innovative and would be utilized in future studies to identify potential functional genes, which can then be used in marker creation, marker-assisted selection, and uncovering the regulatory role of the relevant genes.
| Item Type: | Book Section |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Series Name: | Methods in Molecular Biology |
| Uncontrolled Keywords: | Gene expression, cDNA-SCoT, Fusarium oxysporum, Wilting disease |
| Subjects: | Mandate crops > Chickpea Others > Genetics and Genomics Others > Plant Disease |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 21 Oct 2025 04:05 |
| Last Modified: | 21 Oct 2025 04:05 |
| URI: | http://oar.icrisat.org/id/eprint/13372 |
| Acknowledgement: | We gratefully acknowledge the Biotechnology Centre, Dr. PDKV Akola, for supporting and providing their facilities in this work. In addition, we would like to thank Pulses Research Unit Dr. PDKV, Akola, and ICRISAT, Hyderabad for providing seeds. |
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