Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.)

Priyadarshi, R and Yadav, Shailesh and Pettem, M and Venkateshwarlu, Ch and Pranesh, K J and Singh, V K and Kumar, A (2025) Development of breeding lines with multiple stress tolerance to cold, drought, and high-temperature stress in rice (Oryza sativa L.). Euphytica (TSI), 221. pp. 1-15. ISSN 0014-2336

PDF - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB)

Abstract

Farmers and breeders recognize that multiple abiotic stresses occurring simultaneously are more harmful to crops than individual stress conditions. In this study, pedigree-based breeding was employed to develop multiple abiotic stress-tolerant rice breeding lines (MTU 1010/IRINMST-291), using stringent phenotypic selection for germination and seedling stage cold stress tolerance, reproductive stage drought stress, and reproductive stage high-temperature stress. Among the developed lines, IRINMST-007, IRINMST-338, IRINMST-418, and IRINMST-129 emerged as high-performing families under non-stress, drought, and high-temperature conditions during the reproductive stage. Additionally, IRINMST-007, IRINMST-418, and IRINMST-129 showed strong cold tolerance at the seedling stage and germination stage. These breeding lines have the potential to address food security challenges posed by abiotic stresses. A total of 110 parental polymorphic markers were used for single-marker analysis in the 180 selected F5 recombinant inbred lines. RM5344 were found associated with percent spikelet fertility at reproductive stage high-temperature stress with a phenotypic variance of 24%. RM5911 was found to be associated with grain yield at reproductive stage drought stress with a phenotypic variance of 11%. RM1019 was found associated with seedling growth under cold stress with a phenotypic variance of 18%. RM5344 was found associated with survival rate at seedling stage cold stress with a phenotypic variance of 19%. RM555 was found associated with vigor of germination at germination stage cold stress with a phenotypic variance of 18%. These markers can be used for molecular breeding programs to develop multi-stress tolerant rice varieties.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Cold stress, Drought stress, High-temperature stress, Single marker analysis, Rice breeding
Subjects:	Others > Plant Breeding Others > Rice Others > Drought
Depositing User:	Mr Nagaraju T
Date Deposited:	14 Oct 2025 04:44
Last Modified:	14 Oct 2025 04:44
URI:	http://oar.icrisat.org/id/eprint/13361
Official URL:	https://link.springer.com/article/10.1007/s10681-0...
Projects:	UNSPECIFIED
Funders:	Government of India - Department of Biotechnology
Acknowledgement:	The present study was supported by a grant from the Department of Biotechnology, Government of India (BT/AB/01/IRRI India/2012 dt. 01.07.2013). The authors sincerely thank DBT for their financial support in conducting this study.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item