Saio, Y and Lap, B and Rai, M and Tyagi, W (2025) Molecular and pathotype diversity of Magnaporthe oryzae isolates in NEH region of India and its implications on rice resistance breeding. Indian Phytopathology, 78. pp. 431-443. ISSN 0367-973X
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Abstract
Rice blast, caused by filamentous pathogen Magnaporthe oryzae, is a major peril to rice production, particularly in the North Eastern Hill (NEH) region of India. The dynamics of 24 M. oryzae isolates collected from different geographical locations within the NEH region using 43 SSR markers and pathotyping 17 rice monogenic lines revealed significant genetic diversity. The highest polymorphic information content value of 0.90 was observed for marker mgm285. Five distinct groups were identified with isolate LB 3 collected from upland field in Meghalaya distinct from the rest of the isolates. Significant genetic distinction between isolates from upland and lowland fields, as well as differentiation of tissue infection were observed suggesting the adaptive nature of pathogen and the involvement of specific virulence factors. The highest dissimilarity (0.90) was found between NB 1 and NoB 1 isolates. The virulence frequency varied in the range of 35–76%. The pathotyping of isolates using monogenic lines identified 8 pathotypes, of which pathotype 1 (LB 5 and LB 11) from lowland (Meghalaya) was the most virulent, while pathotype 8 (LB 1) from Nagaland was the least virulent. The data revealed that lowland isolates were more heterogeneous than upland isolates, which had intermediate virulence. In order to prevent future disease outbreaks and to strategically and sustainably breed blast-resistant rice cultivars for NEH region, the profiling M. oryzae isolates is needed along with additional research to unravel the mechanism of host-pathogen interaction via inclusive molecular approaches.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Genetic diversity, Isolates, Pathotyping, Rice blast, SSR markers |
| Subjects: | Others > Genetic Engineering Others > Rice Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 08 Oct 2025 05:12 |
| Last Modified: | 08 Oct 2025 05:12 |
| URI: | http://oar.icrisat.org/id/eprint/13355 |
| Official URL: | https://link.springer.com/article/10.1007/s42360-0... |
| Projects: | UNSPECIFIED |
| Funders: | Government of India - Department of Biotechnology |
| Acknowledgement: | UNSPECIFIED |
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