QTL mapping for yield contributing traits in mungbean (Vigna radiata L.) using a RIL population

D’Agostini, F and Pérez, J R and Madella, M and Vadez, V and Lancelotti, C (2024) QTL mapping for yield contributing traits in mungbean (Vigna radiata L.) using a RIL population. Vegetation History and Archaeobotany, 34. pp. 239-255. ISSN 0939-6314

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Abstract

Mungbean (Vigna radiata L.) is one of the most important yet genomically under-researched leguminous food crop. Its productivity is low due to the complex nature of yield realization, which is regulated by various yield-contributing traits. Thus, understanding the genetic basis of these traits is essential for developing an ideal genotype with high yield. In this study, mapping of quantitative trait loci (QTLs) was conducted for six yield-contributing traits using a recombinant inbred line (RIL) population (166 number), developed by crossing two contrasting genotypes (Pusa Baisakhi × PMR-1). The genotyping-by-sequencing (GBS) of RILs was used to construct the genetic map using 1347 single nucleotide polymorphism (SNPs). The QTL mapping using multiple interval mapping (MIM) and composite interval mapping (CIM) has identified 17 yield contributing QTLs of which, 4 for number of leaves/plant (NL), 3 for plant height (PH), 2 for SPAD value, 3 for 100 seed weight (SW), 2 for number of pods/plant (NP), and 3 for total grain yield (GY). The Logarithm of Odds (LOD) scores for these QTLs ranged from ~ 3–9, while phenotypic variance explained (PVE) ranged from ~ 9–24%. Several candidate genes with mRNA expression and protein-altering mutations were identified as having a direct role in key processes like growth (LOC106756212, LOC106776425, LOC106777991), flowering (LOC106777903, LOC106768860), metabolism (LOC106757749, LOC106758189), etc. The candidate genes are validated through digital gene expression analysis. In addition, Insertion-Deletion (InDel) markers were also developed for the identified QTLs which hold broad applications for the improvement of yield-related traits in mungbean.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Phytoliths, Water availability, Agriculture, Sorghum, Pearl millet, Finger millet
Subjects:	Others > Agriculture Mandate crops > Millets > Pearl Millet Mandate crops > Millets > Finger Millet Mandate crops > Sorghum Others > Water Resources
Depositing User:	Mr Nagaraju T
Date Deposited:	06 Oct 2025 04:34
Last Modified:	06 Oct 2025 04:34
URI:	http://oar.icrisat.org/id/eprint/13353
Official URL:	https://link.springer.com/article/10.1007/s00334-0...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We would like to thank the GEMS team from ICRISAT who helped during the experimental work in the fields. We are also thankful to Jordi Ibañez Insa and Soledad Alvarez of the Geosciences Barcelona (GEO3BCN-CSIC) for conducting the XRF analysis of the sediments. This work is part of the RAINDROPS project funded by the European Research Council (ERC-Stg-2017) under grant agreement n. 759800. University Pompeu Fabra health and safety regulations have been followed during both the fieldwork and the laboratory work. RAINDROPS has received ethical approval from the Institutional Committee for Ethical Review of Projects (CIREP) at Universitat Pompeu Fabra (ethics certificate n. 2017/7662/I). VV was partially supported by the Make Our Planet Great Again (MOPGA) ICARUS project (Improve Crops in Arid Regions and future climates) funded by the Agence Nationale de la Recherche (ANR, grant ANR-17-MPGA-0011). CASEs (UPF) is a Quality Research Group recognised by Agencia de Gestión de Ayudas Universitarias y de Investigación (the Catalan Agency for Research) (AGAUR-SGR 212).
Links:	Google Scholar

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