Multi-Population Analysis for Leaf and Neck Blast Reveals Novel Source of Neck Blast Resistance in Rice

Debnath, A and Sumpi, H and Lap, B and Bhutia, K L and Behera, A and Tyagi, W and Rai, M (2024) Multi-Population Analysis for Leaf and Neck Blast Reveals Novel Source of Neck Blast Resistance in Rice. Plants (TSI), 13 (17). pp. 1-15. ISSN 2223-7747

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Abstract

Rice blast is one of the most devastating biotic stresses that limits rice productivity. The North Eastern Hill (NEH) region of India is considered to be one of the primary centres of diversity for both rice and pathotypes of Magnaporthe grisea. Therefore, the present study was carried out to elucidate the genetic basis of leaf and neck blast resistance under Meghalaya conditions. A set of 80 diverse genotypes (natural population) and 2 F2 populations involving resistant parent, a wildtype landrace, LR 5 (Lal Jangali) and susceptible genotypes Sambha Mahsuri SUB 1 (SMS) and LR 26 (Chakhao Poireiton) were used for association analysis of reported major gene-linked markers with leaf and neck blast resistance to identify major effective genes under local conditions. Genotyping using twenty-five gene-specific markers across diverse genotypes and F2 progenies revealed genes Pi5 and Pi54 to be associated with leaf blast resistance in all three populations. Genes Pib and qPbm showed an association with neck blast resistance in both natural and LR 5 × SMS populations. Additionally, a set of 184 genome-wide polymorphic markers (SSRs and SNPs), when applied to F2-resistant and F2-susceptible DNA bulks derived from LR 5 × LR 26, suggested that Pi20(t) on chromosome 12 is one of the major genes imparting disease resistance. Markers snpOS318, RM1337 and RM7102 and RM247 and snpOS316 were associated with leaf blast and neck blast resistance, respectively. The genotypes, markers and genes will help in marker-assisted selection and development of varieties with durable resistance.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Magnaporthe grisea, blast resistance, association, marker, genotypes, population
Subjects:	Others > Genetic Engineering Others > Rice Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	01 Aug 2025 05:15
Last Modified:	01 Aug 2025 05:15
URI:	http://oar.icrisat.org/id/eprint/13253
Official URL:	https://www.mdpi.com/2223-7747/13/17/2475
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Ng Eng Hwa is acknowledged for facilitating SNP genotyping under CGIAR, HTPG project.
Links:	Google Scholar

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