Debnath, S and Neelam, K and Bhatia, D and Kaur, A and Kumar, Kishor and Singh, K (2025) Marker-Assisted Introgression of qSPP2.2 Loci from Oryza longistaminata Improves Spikelet Number in Basmati Rice. Tropical Plant Biology (TSI), 18. pp. 1-16. ISSN 1935-9756
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Abstract
Basmati rice is the premium, export-quality, long, slender grains with pleasant aroma. Punjab Basmati 3 (PB3), a unique aromatic rice, is sensitive to photoperiod, semi-dwarf, tolerant to lodging, and resistant to bacterial blight (BB). However, it yields less than Indica varieties, mostly due to lower number of grains per panicle (GPP), which varies between 60 and 80. To increase the GPP in PB3, we transferred the grain number QTL qSPP2.2 from O. longistaminata (A. Chev. et Roehr.) derived introgression line, RIL127 into PB3 using marker-assisted selection (MAS). Marker- assisted foreground selection using polymorphic markers linked to the qSPP2.2 loci in plants from backcross progenies and their advanced self-progenies, coupled with their background analysis for BB resistance (Xa21, xa13), Basmati aroma (Badh2) and intermediate amylose content (wx locus) and analysis of their grain quality characteristics ensured an efficient and successful introgression of the QTL qSPP2.2 into PB3. The phenotypic expression of qSPP2.2 in the Basmati background was studied in BC1F2, BC2F3 and their advanced selfed progenies, and it shows a 35–40% increase in the grain number of PB3, demonstrating a significant improvement of grain yield of the introgressed lines. Together, a large number of backcross progenies and their advanced self-progenies, coupled with MAS for desirable alleles, and extensive phenotyping have resulted in an improved version of PB3 with higher spikelet per panicle (SPP). Correlation analysis using 384 replicated F2 plants revealed no significant correlation between SPP and grain length, however, a significant weak negative correlation (-0.150*) was observed between SPP and grain breadth, suggesting the possibility of raising the GPP and the total output of Basmati rice having exceptional quality.
| Item Type: | Article |
|---|---|
| Divisions: | Genebank |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Marker-assisted backcrossing, Markers-assisted selection, Oryza longistaminata, Punjab Basmati 3, qSPP2.2, Spikelet per panicle |
| Subjects: | Others > Rice Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 18 Jun 2025 08:39 |
| Last Modified: | 18 Jun 2025 08:39 |
| URI: | http://oar.icrisat.org/id/eprint/13169 |
| Official URL: | https://link.springer.com/article/10.1007/s12042-0... |
| Projects: | Molecular Breeding for improvement of tolerance to biotic and abiotic stresses, yield and quality traits in crops (Rice component)” ICAR-85 (PC-2330)) |
| Funders: | Indian Council of Agricultural Research |
| Acknowledgement: | The authors would like to thank Indian Council of Agricultural Research (ICAR), New Delhi, India for providing necessary funds and fellowship grant. The authors are also thankful to Punjab Agricultural University and Molecular Biology Laboratories of Gurdev Singh Khush Lab of School of Agricultural Biotechnology, PAU, Ludhiana, Punjab, India for availing necessary facilities and instrumentation. Thanks to the National Rice Research Institute, Cuttack, Odisha, India for availing farm land and other necessary resources for off-season cultivation of rice. We are also highly thankful to our technical staffs and skilled labors for their tireless support throughout the cropping seasons. |
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