Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis

Awata, L A O and Ifie, B E and Danquah, E and Tongoona, P and Suresh, L M and Gowda, Manje and Marchelo-Dragga, P W and Sitonik, C and Olsen, M and Prasanna, B M and Jumbo, M B (2021) Comparison of non-overlapping maize populations of unequal sizes for resistance to maize lethal necrosis. Journal of Crop Improvement (TSI), 36 (4). pp. 494-513. ISSN 1542-7528

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Abstract

Contrast between marker-assisted backcross (MABC) and doubled haploid (DH) methods in transferring genes for resistance to maize lethal necrosis (MLN) in maize (Zea mays L.) is not well understood. The MLN is caused by co-infection of maize plant by maize chlorotic mottle virus and sugarcane mosaic virus. Two maize panels consisting of four BC3F2 and six DH populations, separately developed through marker-assisted selection from crosses between susceptible CIMMYT lines and MLN-resistant donor parent (KS23-6), were used in the current study. The two populations were of different population structures with unequal sizes. Experiments were conducted under artificial MLN inoculations for two seasons in 2018. Analyses of variance revealed significant variations among genotypes in both panels (p ≤ 0.001). Levene’s and Welch’s tests found that variances and means of the BC3F2 and DH populations were highly unequal (p ≤ 0.001). The study identified genotypes with reduced MLN infections in both populations; however, lower means for MLN severity and area under disease progress curve (AUDPC) values, and higher heritability estimates were obtained in the DH populations than in the BC3F2 populations. Additionally, the DH populations showed higher relative genetic gains for resistance to MLN compared with the BC3F2 populations. The current study detected superiority of DH over MABC populations for breeding for resistance to MLN. Nevertheless, the results observed in the present study warrant further investigations using the same genetic materials with identical population sizes.

Item Type:	Article
Divisions:	Research Program : East & Southern Africa
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Backcross, doubled-haploid lines, lethal necrosis, marker-assisted selection, zea mays
Subjects:	Others > Genetics and Genomics Others > Maize
Depositing User:	Mr Nagaraju T
Date Deposited:	11 Jun 2025 05:47
Last Modified:	11 Jun 2025 05:47
URI:	http://oar.icrisat.org/id/eprint/13140
Official URL:	https://www.tandfonline.com/doi/full/10.1080/15427...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors thank The Global maize Program, CIMMYT for providing genetic materials, technical support, and access to MLN facilities for conducting the experiments, with support from the Bill and Melinda Gates Foundation. They also thank the reviewers for their valuable suggestions and corrections and the active role of the editor(s) during the revision of our manuscript. This research was part of the PhD thesis of the first author, funded by Intra-ACP CSAA Mobility Schemes at West Africa Center for Crop Improvement (WACCI), University of Ghana, Legon, Ghana.
Links:	Google Scholar

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