Zhao, C and He, L and Xia, H and Zhou, X and Geng, Y and Hou, L and Li, P and Li, G and Zhao, S and Ma, C and Tang, R and Pandey, M K and Varshney, R K and Wang, X (2021) De novo full length transcriptome analysis of Arachis glabrata provides insights into gene expression dynamics in response to biotic and abiotic stresses. Genomics, 113 (3). pp. 1579-1588. ISSN 0888-7543
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Abstract
The perennial ornamental peanut Arachis glabrata represents one of the most adaptable wild Arachis species. This study used PacBio combined with BGISEQ-500 RNA-seq technology to study the transcriptome and gene expression dynamics of A. glabrata. Of the total 109,747 unique transcripts obtained, >90,566 transcripts showed significant homology to known proteins and contained the complete coding sequence (CDS). RNA-seq revealed that 1229, 1039, 1671, 3923, 1521 and 1799 transcripts expressed specifically in the root, stem, leaf, flower, peg and pod, respectively. We also identified thousands of differentially expressed transcripts in response to drought, salt, cold and leaf spot disease. Furthermore, we identified 30 polyphenol oxidase encoding genes associated with the quality of forage, making A. glabrata suitable as a forage crop. Our findings presented the first transcriptome study of A. glabrata which will facilitate genetic and genomics studies and lays the groundwork for a deeper understanding of the A. glabrata genome.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Arachis glabrata, Peanut, Full-length transcriptome, PacBio, RNA-seq, Stresses |
| Subjects: | Others > Abiotic Stress Others > Biotic Stress Mandate crops > Groundnut Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 03 Jun 2025 04:04 |
| Last Modified: | 03 Jun 2025 04:04 |
| URI: | http://oar.icrisat.org/id/eprint/13101 |
| Official URL: | https://www.sciencedirect.com/science/article/pii/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | Funding This research is supported by Natural Science Foundation of China (32072090, 31861143009, 31801301), High-level Foreign Experts Introduction Program of China (GL20200123001), Key Research and Development Project of Shandong Province (2020LZGC001), Guangxi Natural Science Foundation (2018GXNSFAA281079), Shandong Provincial Crop Elite Variety Development Project, Taishan Scholar Project of Shandong Province (ts20190964) and Agricultural Scientific and Technological Innovation Project of SAAS (CXGC2018E13, CXGC2016B02). |
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