Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm

Bohra, A and Prasad, G and Rathore, A and Saxena, R K and Naik, S J S and Pareek, S and Jha, R and Pazhamala, L T and Datta, D and Pandey, G and Tiwari, A and Maurya, A K and Soren, K R and Akram, M and Varshney, R K and Singh, N P (2021) Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm. The Plant Genome (TSI), 14 (3). pp. 1-15. ISSN 1940-3372

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Abstract

Cytoplasmic male sterility(CMS), a maternally inherited trait, provides a promising means to harness yield gains associated with hybrid vigor. In pigeonpea [Cajanus cajan (L.) Huth], nine types of sterility-inducing cytoplasm have been reported, of which A2 and A4 have been successfully deployed in hybrid breeding. Unfortunately, molecular mechanism of the CMS trait is poorly understood because of limited research invested. More recently, an association between a mitochondrial gene (nad7) and A4-CMS has been demonstrated in pigeonpea; however, the mechanism underlying A2-CMS still remains obscure. The current investigation aimed to analyze the differences in A2-CMS line (ICPL 88039A) and its isogenic maintainer line (ICPL 88039B) at transcriptome level using next-generation sequencing. Gene expression profiling uncovered a set of 505 genes that showed altered expression in response to CMS, of which, 412 genes were upregulated while 93 were downregulated in the fertile maintainer line vs. the CMS line. Further, gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses revealed association of CMS in pigeonpea with four major pathways: glucose and lipid metabolism, ATP production, pollen development and pollen tube growth, and reactive oxygen species (ROS) scavenging. Patterns of digital gene expression were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) of six candidate genes. This study elucidates candidate genes and metabolic pathways having potential associations with pollen development and male sterility in pigeonpea A2-CMS. New insights on molecular mechanism of CMS trait in pigeonpea will be helpful to accelerate heterosis utilization for enhancing productivity gains in pigeonpea.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Cytoplasmic male sterility(CMS), A2 cytoplasm, yield gains, pigeonpea
Subjects:	Mandate crops > Pigeonpea Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	03 Jun 2025 03:33
Last Modified:	03 Jun 2025 03:33
URI:	http://oar.icrisat.org/id/eprint/13099
Official URL:	https://acsess.onlinelibrary.wiley.com/doi/full/10...
Projects:	UNSPECIFIED
Funders:	Indian Council of Agri-cultural Research (ICAR), Bill and Melinda Gates Foundation
Acknowledgement:	AB acknowledges financial support Indian Council of Agricultural Research (ICAR), New Delhi (Grant No. AGENIAS-RICOP201501000047). RKV thanks Bill and Melinda Gates Foundation for support.
Links:	Google Scholar

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