Li, W and Huang, L and Liu, N and Pandey, M K and Chen, Y and Cheng, L and Guo, J and Yu, B and Luo, H and Zhou, X and Huai, D and Chen, W and Yan, L and Wang, X and Lei, Y and Varshney, R K and Liao, B and Jiang, H (2021) Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts. International Journal of Molecular Sciences (TSI), 22 (14). pp. 1-20. ISSN 1661-6596
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Abstract
Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | transcriptional regulation, sucrose metabolism, RNA-based sequencing (RNA-seq), development, peanut seed |
| Subjects: | Mandate crops > Groundnut Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 02 Jun 2025 08:27 |
| Last Modified: | 02 Jun 2025 08:27 |
| URI: | http://oar.icrisat.org/id/eprint/13095 |
| Official URL: | https://www.mdpi.com/1422-0067/22/14/7266 |
| Projects: | National Program for Crop Germplasm Protection of China, Open Project of the Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P.R. China |
| Funders: | National Peanut Industry Technology System Construction, National Crop Germplasm Resources Center |
| Acknowledgement: | The authors are indebted to Xiaomeng Xue and Jie Liu (Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences) for assistance with the qRT-PCR analysis, Libing Li (Hubei University) for guidance of the WGCNA, and Meiling Hu (Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences) for detection of the oil content. |
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