Molecular characterization of Indian Dolichos bean {Lab/ab purpureus L. var. typicus Prain) accessions using RAPD markers

Pidigam, S and Munnam, S and Nimmarajula, S and Amarapalli, G and Sudini, H K and Pandravada, S R (2021) Molecular characterization of Indian Dolichos bean {Lab/ab purpureus L. var. typicus Prain) accessions using RAPD markers. Indian Journal of Genetics and Plant Breeding, 81 (2). pp. 322-326. ISSN 0975-6906

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Abstract

In this study, 29 genotypes of (Lab/ab purpureus) Indian bean augmented from different geographical regions were analyzed for genetic diversity using 39 RAPDs. The binary matrix data revealed 97 .4% polymorphism among genotypes, with 2322 amplification fragments. The PIC values ranged from 0.25 to 0.9 6 with an average of O. 70. Markers OPA05, OPB15, and OPV12 showed high discrimination among all the genotypes. A magnificent range of Jaccard's genetic similarity coefficient was evaluated and UPGMA based dendrogram classified genotypes into 4 major clusters. lC-384066 and NSJ/NAIP/ 192 were found to be unique and highly diversified. The same grouping patterns were also observed in the modelbased STRUCTURE analysis (K=2). The study showed the degree and distribution of genetic diversity in Dolichos bean, which can be utilized to identify the parental lines, to develop mapping populations and breeding.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Dolichos bean, genetic diversity, RAPD, STRUCTURE, UPGMA
Subjects:	Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	07 May 2025 08:06
Last Modified:	07 May 2025 08:06
URI:	http://oar.icrisat.org/id/eprint/13068
Official URL:	https://isgpb.org/journal/index.php/IJGPB/article/...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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