Natural polymorphisms in a pair of NSP2 homoeologs can cause loss of nodulation in peanut

Peng, Z and Chen, H and Tan, L and Shu, H and Varshney, R K and Zhou, Z and Zhao, Z and Luo, Z and Chitikineni, A and Wang, L and Maku, J and López, Y and Gallo, M and Zhou, H and Wang, J (2020) Natural polymorphisms in a pair of NSP2 homoeologs can cause loss of nodulation in peanut. Journal of Experimental Botany (TSI), 72 (4). pp. 1104-1118. ISSN 0022-0957

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Abstract

Microbial symbiosis in legumes is achieved through nitrogen-fixing root nodules, and these are important for sustainable agriculture. The molecular mechanisms underlying development of root nodules in polyploid legume crops are largely understudied. Through map-based cloning and QTL-seq approaches, we identified a pair of homoeologous GRAS transcription factor genes, Nodulation Signaling Pathway 2 (AhNSP2-B07 or Nb) and AhNSP2-A08 (Na), controlling nodulation in cultivated peanut (Arachis hypogaea L.), an allotetraploid legume crop, which exhibited non-Mendelian and Mendelian inheritance, respectively. The segregation of nodulation in the progeny of Nananbnb genotypes followed a 3:1 Mendelian ratio, in contrast to the 5:3~1:1 non-Mendelian ratio for nanaNbnb genotypes. Additionally, a much higher frequency of the nb allele (13%) than the na allele (4%) exists in the peanut germplasm collection, suggesting that Nb is less essential than Na in nodule organogenesis. Our findings reveal the genetic basis of naturally occurred non-nodulating peanut plants, which can be potentially used for nitrogen fixation improvement in peanut. Furthermore, the results have implications for and provide insights into the evolution of homoeologous genes in allopolyploid species.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Allotetraploid, GRAS transcription factor, nodulation, non-Mendelian inheritance, NSP2, peanut, symbiosis
Subjects:	Mandate crops > Groundnut Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	17 Apr 2025 06:35
Last Modified:	17 Apr 2025 06:35
URI:	http://oar.icrisat.org/id/eprint/13039
Official URL:	https://academic.oup.com/jxb/article/72/4/1104/594...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This study was funded by the Major Program of Guangdong Basic and Applied Research (2019B030302006), Florida Peanut Producers Association, the University of Florida IFAS Early Career Scientists Award, and Guangdong Natural Science Funds for Distinguished Young Scholars (Grant No. 2017A030306001). We thank Dr Zhonglin Mou for providing modified pCAMBIA1300 vector. The authors declare no conflict of interest.
Links:	Google Scholar

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