Louis, Y and Moses, M and Michael, C and Davis, G and Abel, S and Lawrent, P and Vicki, M and Peter, S and Wisdom, C (2024) Diversity among Bambara groundnut (Vigna subterranea L. Verdc) accessions using agro-morphological traits and diversity array technologies sequence low density markers in Malawi. Genetic Resources and Crop Evolution (TSI). pp. 1-15. ISSN 0925-9864
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Abstract
Bambara groundnut (Vigna subterranea (L.) Verdc) is a neglected and underutilized crop that plays a big role in improving livelihoods of smallholder farmers in Sub-Saharan Africa. Despite its importance, there is limited availability of commercially improved cultivars to smallholder farmers in Malawi. This study characterized selected Bambara groundnuts accessions for agro-morphological traits for germplasm discrimination. It also identified genetic variation using Single Nucleotide Polymorphism (SNP) markers through Diversity Array Technologies Sequence Low Density (DArTseqLD) that could be used to produce improved seed for crop improvement. Forty Bambara groundnuts accessions were evaluated at the Crops and Soil Sciences Department’s farm of Lilongwe University of Agriculture and Natural Resources, Bunda College, Malawi. From the 40 accessions, 188 unique seed samples were selected for genotyping using DArTseqLD SNP markers. Data on agro-morphological traits were collected following the Bambara groundnut descriptor guidelines and multivariate analysis were performed. Principal Component Analysis revealed a total variation of 53%. The study generated 1048 DArTseqLD SNP markers. Analysis of molecular variance (AMOVA) identified 84% and 13% of genetic variation among and within the Bambara groundnut accessions respectively, whereas 3% genetic variation was observed among the total populations. Cluster analysis based on genotypic data grouped the 188 samples into 10 clusters. Based on phenotypic and genotypic data, it can be concluded that there is a significant degree of variation and genetic diversity in the accessions evaluated that can be used in crop improvement program as well as being directly used by farmers in seed production.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : East & Southern Africa |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Bambara groundnut, Accessions, Smallholder farmers, Multivariate analysis, Phenotyping, Genotyping |
| Subjects: | Others > Smallholder Farmers Mandate crops > Groundnut Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 25 Feb 2025 04:34 |
| Last Modified: | 25 Feb 2025 04:34 |
| URI: | http://oar.icrisat.org/id/eprint/12979 |
| Official URL: | https://link.springer.com/article/10.1007/s10722-0... |
| Projects: | Research Project on Seed Systems |
| Funders: | The McKnight foundation Collaborative Crop Research Program |
| Acknowledgement: | The authors are thankful to the McKnight foundation through its Collaborative Crop Research Program (CCRP) that funded the Seed Systems’ research project (Grant no. 19-265). Thankful to all collaborators and supervisors for technical support at the time of project implementation. I would like to acknowledge Frank Tchuwa and Daimon Kambewa for their technical support during the project implementation. We would also like to acknowledge Ric Coe and the entire CCRP team for their technical support during data processing and analysis and not forgetting farmers who provided the germplasms used in this study. |
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