The auxin efflux carrier PIN1a regulates vascular patterning in cereal roots

Fusi, R and Milner, S G and Rosignoli, S and Bovina, R and Teixeira, C D J V and Lou, H and Atkinson, B S and Borkar, A N and York, L M and Jones, D H and Sturrock, C J and Stein, N and Mascher, M and Tuberosa, R and O’Connor, D and Bennett, M J and Bishopp, A and Salvi, S and Bhosale, R (2024) The auxin efflux carrier PIN1a regulates vascular patterning in cereal roots. New Phytologist, 244 (1). pp. 104-115. ISSN 0028-646X

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Abstract

Barley (Hordeum vulgare) is an important global cereal crop and a model in genetic studies. Despite advances in characterising barley genomic resources, few mutant studies have identified genes controlling root architecture and anatomy, which plays a critical role in capturing soil resources. Our phenotypic screening of a TILLING mutant collection identified line TM5992 exhibiting a short-root phenotype compared with wild-type (WT) Morex background. Outcrossing TM5992 with barley variety Proctor and subsequent SNP array-based bulk segregant analysis, fine mapped the mutation to a cM scale. Exome sequencing pinpointed a mutation in the candidate gene HvPIN1a, further confirming this by analysing independent mutant alleles. Detailed analysis of root growth and anatomy in Hvpin1a mutant alleles exhibited a slower growth rate, shorter apical meristem and striking vascular patterning defects compared to WT. Expression and mutant analyses of PIN1 members in the closely related cereal brachypodium (Brachypodium distachyon) revealed that BdPIN1a and BdPIN1b were redundantly expressed in root vascular tissues but only Bdpin1a mutant allele displayed root vascular defects similar to Hvpin1a. We conclude that barley PIN1 genes have sub-functionalised in cereals, compared to Arabidopsis (Arabidopsis thaliana), where PIN1a sequences control root vascular patterning.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	auxin efflux carrier, barley, PIN1, root, vascular development
Subjects:	Others > Cereals
Depositing User:	Mr Nagaraju T
Date Deposited:	13 Nov 2024 05:39
Last Modified:	13 Nov 2024 05:39
URI:	http://oar.icrisat.org/id/eprint/12858
Official URL:	https://nph.onlinelibrary.wiley.com/doi/full/10.11...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We acknowledge the technical support of Simona Corneti, Anne Fiebig, Sandra Stefanelli. RF acknowledges the Hounsfield Facility Center at the University of Nottingham for all the support provided for this work. The Hounsfield Facility received funding from European Research Council (Futureroots Project, Grant agreement ID: 294729), Biotechnology and Biological Sciences Research Council of the United Kingdom and The Wolfson Foundation. HL was supported by a joint University of Adelaide – University of Nottingham PhD scholarship. This manuscript has been authored in part by LMY at UT-Battelle, LLC, under contract DE-AC05-00OR22725 with the US Department of Energy (DOE). The publisher acknowledges the US government licence to provide public access under the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). R. Bhosale acknowledges support from BBSRC Discovery Fellowship (BB/S011102/1), BBSRC New Investigator grant (BB/X014843/1), and BBSRC-funded Delivering Sustainable Wheat (DSW) Partner Grant (BB/X018806/1).
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