Phenotyping and a genome-wide association study of elite lines of pearl millet

Kambara, K and Gupta, S K and Takano, T and Tsugama, D (2024) Phenotyping and a genome-wide association study of elite lines of pearl millet. Breeding Science, 74. pp. 240-246. ISSN 1347-3735

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Abstract

Pearl millet (Pennisetum glaucum (L.) R. BR.) is a cereal crop mainly grown in India and sub-Saharan Africa. In pearl millet, genes and genomic regions associated with traits are largely unknown. Pearl millet parental lines bred at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) are useful for the production and breeding of pearl millet. However, the phenotypic diversity of these lines has not been fully evaluated. In this study, 16 traits of 107 of those parental lines were assessed with field trials in Japan, and a genome-wide association study (GWAS) was performed using these phenotypic data to identify the genomic regions and genes associated with those traits. The GWAS revealed genomic regions associated with culm height and pigmentation of the shoot basal part (PS). The genomic region associated with PS contained a homolog of PHENYLALANINE AMMONIA LYASE 2 (PAL2), a gene involved in anthocyanin accumulation in Arabidopsis thaliana. The PAL2 homolog can be a candidate for a gene involved in regulating PS in pearl millet. These results provide a better understanding of the phenotypic diversity of pearl millet and its genetic background.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	pearl millet, Pennisetum glaucum, elite lines, GWAS, PAL2
Subjects:	Mandate crops > Millets > Pearl Millet Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	22 Aug 2024 05:04
Last Modified:	22 Aug 2024 05:04
URI:	http://oar.icrisat.org/id/eprint/12784
Official URL:	https://www.jstage.jst.go.jp/article/jsbbs/74/3/74...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We are grateful to ICRISAT for providing the pearl millet seeds for this study. We thank the technical staff of the Institute for Sustainable Agroecosystem Services, The University of Tokyo, for preparing the fields used in this study. This study was financially supported by JSPS (Japan Society for the Promotion of Science) KAKENHI Grant [grant number JP 19KK0155], and by Cabinet Office, Government of Japan, Moonshot Research and Development Program for Agriculture, Forestry and Fisheries (funding agency: Bio-oriented Technology Research Advancement Institution) [grant number JPJ009237].
Links:	Google Scholar

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