Genome‑wide screening and characterization of phospholipase A (PLA)‑like genes in sorghum (Sorghum bicolor L.)

Sapara, V J and Shankhapal, A R and Reddy, P S (2024) Genome‑wide screening and characterization of phospholipase A (PLA)‑like genes in sorghum (Sorghum bicolor L.). Planta, 260. pp. 1-13. ISSN 0032-0935

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Abstract

Sorghum bicolor (L.) Moench is the fifth most cultivated crop worldwide, and it is used in many ways, but it has always gained less popularity due to the yield, pest, and environmental constraints. Improving genetic background and developing better varieties is crucial for better sorghum production in semi-arid tropical regions. This study focuses on the phospholipase A (PLA) family within sorghum, comprehensively characterising PLA genes and their expression across different tissues. The investigation identified 32 PLA genes in the sorghum genome, offering insights into their chromosomal localization, molecular weight, isoelectric point, and subcellular distribution through bioinformatics tools. PLA-like family genes are classified into three groups, namely patatin-related phospholipase A (pPLA), phospholipase A1 (PLA1), and phospholipase A2 (PLA2). In-silico chromosome localization studies revealed that these genes are unevenly distributed in the sorghum genome. Cis-motif analysis revealed the presence of several developmental, tissue and hormone-specific elements in the promoter regions of the PLA genes. Expression studies in different tissues such as leaf, root, seedling, mature seed, immature seed, anther, and pollen showed differential expression patterns. Taken together, genome-wide analysis studies of PLA genes provide a better understanding and critical role of this gene family considering the metabolic processes involved in plant growth, defence and stress response.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Bioinformatics tools, Cis-motif, Chromosomal localization, Gene expression studies, In-silico, Metabolic process, Sorghum genome
Subjects:	Mandate crops > Sorghum Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	04 Jul 2024 08:17
Last Modified:	04 Jul 2024 08:17
URI:	http://oar.icrisat.org/id/eprint/12737
Official URL:	https://link.springer.com/article/10.1007/s00425-0...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	All authors sincerely thank ICRISAT for providing financial assistance and support for conducting research. VS acknowledges the DST-INSPIRE fellowship (DST/INSPIRE Fellowship/IF190079) from the Department of Science and Technology, Government of India for her doctoral research work.
Links:	Google Scholar

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