Bhatnagar-Mathur, P and Yogendra, K and Parankusam, S and Sanivarapu, H and Prasad, K and Lingampali, S B and Sharma, K K (2021) Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.). Journal of Plant Interactions, 16 (1). pp. 494-509. ISSN 1742-9145
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Abstract
Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic analysis was performed in two contrasting peanut genotypes, variety JL 24 (susceptible) and its near-isogenic resistant transgenic derivative expressing an alfalfa defensin gene. Several resistance proteins associated with secondary metabolic pathways were strongly induced in the resistant genotypes including phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, chalcone synthase, resveratrol synthase, flavanone-3-hydroxylase, lipoxygenase, diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase, β-ketoacyl-ACP-reductase, monoacylglycerol acyltransferase, and diacylglycerol acyltransferase, indicating their roles in resistance. Besides, several putative susceptibility-associated proteins were revealed providing knowledge on potential candidate target genes for precise breeding interventions for aflatoxin mitigation. This is the first study to demonstrate comparative proteomics analysis in Aspergillus–peanut interaction using contrasting near-isogenic lines to elucidate the underlying molecular mechanisms of resistance.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Genetic Gains |
| CRP: | CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC) |
| Uncontrolled Keywords: | Aflatoxin, Arachis hypogaea, Aspergillus flavus, flavonoids, peanut, proteomics, phenylpropanoids |
| Subjects: | Mandate crops > Groundnut Others > Aflatoxins |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 21 Jun 2024 05:38 |
| Last Modified: | 21 Jun 2024 05:38 |
| URI: | http://oar.icrisat.org/id/eprint/12716 |
| Official URL: | https://www.tandfonline.com/doi/full/10.1080/17429... |
| Projects: | UNSPECIFIED |
| Funders: | Government of India - Department of Biotechnology |
| Acknowledgement: | Authors thank Pradeep Reddy, B. Brahmananda Reddy, and N. Kedarinath for assistance with various experiments. |
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