Aerially Applied Zinc Oxide Nanoparticle Affects Reproductive Components and Seed Quality in Fully Grown Bean Plants (Phaseolus vulgaris L.)

    Salehi, H and Rad, A C and Sharifan, H and Raza, A and Varshney, R K (2022) Aerially Applied Zinc Oxide Nanoparticle Affects Reproductive Components and Seed Quality in Fully Grown Bean Plants (Phaseolus vulgaris L.). Frontiers in Plant Science (TSI), 12. pp. 1-13. ISSN 1664-462X

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    Abstract

    The development of reproductive components in plant species is susceptible to environmental stresses. The extensive application of zinc oxide nanoparticles (nZnO) in various agro-industrial processes has jeopardized the performance and functionality of plants. To understand the response of the developmental (gametogenesis and sporogenesis) processes to nanoparticles (NPs) exposure, the aerial application of nZnO and their ionic counterpart of ZnSO4 at four different levels were examined on bean plants (Phaseolus vulgaris) before the flowering stage. To evaluate the mentioned processes, briefly, flowers in multiple sizes were fixed in paraffin, followed by sectioning and optical analysis. The possibility of alteration in reproductive cells was thoroughly analyzed using both light and electron microscopes. Overall, our results revealed the histological defects in male and female reproductive systems of mature plants depend on NPs levels. Furthermore, NPs caused tapetum abnormalities, aberrations in carbohydrate accumulation, and apoptosis. The nZnO induced abnormal alterations right after meiosis and partly hindered the microspore development, leading to infertile pollens. The seed yield and dry weight were reduced to 70 and 82% at 2,000 mg L–1 nZnO foliar exposure, respectively. The sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis pattern showed the increased expression of two proteins at the molecular weight of 28 and 42 kDa at various concentrations of nZnO and ZnSO4. Overall, our results provided novel insights into the negative effect of nano-scaled Zn on the differential mechanism involved in the reproductive stage of the plants compared with salt form.

    Item Type: Article
    Divisions: Center of Excellence in Genomics and Systems Biology
    CRP: UNSPECIFIED
    Uncontrolled Keywords: reproductive components, pollen dysfunction, seed productivity, food quality, nanotechnology
    Subjects: Others > Food and Nutrition
    Depositing User: Mr Nagaraju T
    Date Deposited: 23 Apr 2024 10:44
    Last Modified: 23 Apr 2024 10:44
    URI: http://oar.icrisat.org/id/eprint/12647
    Official URL: https://www.frontiersin.org/journals/plant-science...
    Projects: Tropical Legumes III project
    Funders: Bill and Melinda Gates Foundation
    Acknowledgement: ACR is thankful to Bu-Ali Sina University for supporting this research. RKV is grateful to the Bill & Melinda Gates Foundation for supporting legumes research at ICRISAT through Tropical Legumes III project (OPP1114827) and also for supporting this research in part.
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