Discovery of Major Quantitative Trait Loci and Candidate Genes for Fresh Seed Dormancy in Groundnut

Bomireddy, D and Gangurde, S S and Variath, M T and Janila, P and Manohar, S S and Sharma, V and Parmar, S and Deshmukh, D and Reddisekhar, M and Reddy, D M and Sudhakar, P and Reddy, B V B and Varshney, R K and Guo, B and Pandey, M K (2022) Discovery of Major Quantitative Trait Loci and Candidate Genes for Fresh Seed Dormancy in Groundnut. Agronomy (TSI), 12 (2). pp. 1-18. ISSN 2073-4395

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Abstract

Spanish bunch groundnut varieties occupy most of the cultivated area in Asia and Africa, and these varieties lack required 2-3 weeks of fresh seed dormancy (FSD) hampering kernel quality. Genomic breeding can help to improve commercial groundnut cultivars for FSD in a shorter time with greater precision. In this regard, a recombinant inbred line (RIL) population from the cross ICGV 02266 (non-dormant) × ICGV 97045 (dormant) was developed and genotyped with a 5 K mid-density genotyping assay. A linkage map was constructed with 325 SNP loci spanning a total map length of 2335.3 cM and five major QTLs were identified on chromosomes Ah01, Ah11, Ah06, Ah16 and Ah17. Based on differential gene expression using transcriptomic information from dormant (Tifrunner) and non-dormant (ICGV 91114) genotypes, histone deacetylases, histone-lysine N-methyltransferase, cytochrome P450, protein kinases, and ethylene-responsive transcription factor were identified as key regulators involved in the hormonal regulation of dormancy. Six Kompetitive Allele Specific PCR (KASP) markers were successfully validated in the diverse panel including selected RILs of the same population and germplasm lines. These validated KASP markers could facilitate faster breeding of new varieties with desired dormancy using marker-assisted early generation selection.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	genetic map, KASP markers, mid-density genotyping assay, pre-harvest sprouting
Subjects:	Mandate crops > Groundnut Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	26 Mar 2024 03:37
Last Modified:	26 Mar 2024 03:37
URI:	http://oar.icrisat.org/id/eprint/12598
Official URL:	https://www.mdpi.com/2073-4395/12/2/404
Projects:	UNSPECIFIED
Funders:	National Agricultural Science Fund (NASF) of the Indian Council of Agricultural Research (ICAR), India.
Acknowledgement:	The authors thank the National Agricultural Science Fund (NASF) of the Indian Council of Agricultural Research, India for partial financial assistance in this study. Deekshitha Bomireddy acknowledges Acharya N.G. Ranga Agricultural University for collaborating with ICRISAT and the opportunity given as a student to pursue this investigation at ICRISAT. The work was carried out as part of CRP-GLDC. The authors are thankful for the support from OFID for the population development and phenotyping work.
Links:	Google Scholar

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