Nitnavare, R B and Pothana, A and Yeshvekar, R K and Bhattacharya, J and Sapara, V and Reddy, P S and Ramtirtha, Y and Tarafdar, A and Sharma, M and Bhatnagar-Mathur, P (2022) Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil-Borne Chickpea Fungal Pathogens. Journal of Plant Growth Regulation, 42. pp. 6244-6260. ISSN 1435-8107
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Abstract
Defensins are broad-spectrum antimicrobial peptides that play an important role in providing innate immunity to various biotic stresses in plants. We identified and characterized 22 defensin (DEF) and defensin-like (DEFL) genes in chickpea (Cicer arientinum) based on their structures, expression, chromosomal localization, conserved motifs, and cis-regulatory elements. The localization of DEF and DEFL genes in chickpea genome revealed the presence of at least two clusters that are likely evolved through local gene duplications. Genotype-specific responses of several CaDEF and CaDEFL genes in fungal bioassays suggested their involvement in defense against fungal pathogens such as hemi-biotrophic F. oxysporum f. sp. ciceris and dry root rot causing necrotrophic R. bataticola. Molecular docking studies revealed interactions of CaDEFs with fungal plasma membrane components such as phosphatidylserine (PS) and glucosylceramide (GluCer) and their binding sites were identified. Our data will be useful to identify potential candidate genes and their role in host-plant resistance in chickpea, besides presenting opportunities for their potential for possible deployment in other crops.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Anti-fungal proteins (AFPs), Chickpea, Defensins, dry root rot, Fusarium, Host pathogen interactions |
| Subjects: | Others > Genetic Engineering Mandate crops > Chickpea |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 21 Feb 2024 10:54 |
| Last Modified: | 21 Feb 2024 10:54 |
| URI: | http://oar.icrisat.org/id/eprint/12497 |
| Official URL: | https://link.springer.com/article/10.1007/s00344-0... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | All authors express sincere thanks to these funding agencies for providing financial assistance, support for conducting research and other activities at ICRISAT. ICRISAT is a part of CGIAR consortium. Authors also express their sincere thanks to, Mr. Rameshwar Telangare and Mr. B Pradeep Reddy. AP acknowledges DST INSPIRE fellowship received from Department of Science and Technology, Government of India. |
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