Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Kumar, M and Prusty, M R and Pandey, M K and Singh, P K and Bohra, A and Guo, B and Varshney, R K (2023) Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants. Frontiers in Plant Science (TSI), 14. 01-22. ISSN 1664-462X

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Abstract

Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches and transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery of engineered nucleases as genetic scissors to carry out precise manipulation in crop stress-responsive genes and associated molecular network has paved the way for sustainable management of abiotic stress conditions. In this context, the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool has revolutionized due to its simplicity, accessibility, adaptability, flexibility, and wide applicability. This system has great potential to build up crop varieties with enhanced tolerance against abiotic stresses. In this review, we summarize the latest findings on understanding the mechanism of abiotic stress response in plants and the application of CRISPR/Cas-mediated gene-editing system towards enhanced tolerance to a multitude of stresses including drought, salinity, cold, heat, and heavy metals. We provide mechanistic insights on the CRISPR/Cas9-based genome editing technology. We also discuss applications of evolving genome editing techniques such as prime editing and base editing, mutant library production, transgene free and multiplexing to rapidly deliver modern crop cultivars adapted to abiotic stress conditions.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	abiotic stress tolerance, base editing, CRISPR/Cas9, crop production, gene editing, prime editing
Subjects:	Others > Abiotic Stress Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	13 Feb 2024 05:29
Last Modified:	13 Feb 2024 05:29
URI:	http://oar.icrisat.org/id/eprint/12463
Official URL:	https://www.frontiersin.org/journals/plant-science...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	RKV thanks the Food Futures Institute of Murdoch University for the start-up grant, and BG thanks National Peanut Board, Georgia Peanut Commission and USDA Agriculture Research Service for financial support to this study and publication charges of the article.
Links:	Google Scholar

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