Kudapa, H and Ghatak, A and Barmukh, R and Chaturvedi, P and Khan, A and Kale, S and Fragner, L and Chitikineni, A and Weckwerth, W and Varshney, R K (2023) Integrated multi-omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.). The Plant Genome (TSI), SE. pp. 1-17. ISSN 1940-3372
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Abstract
Drought is one of the major constraints limiting chickpea productivity. To unravel complex mechanisms regulating drought response in chickpea, we generated transcriptomics, proteomics, and metabolomics datasets from root tissues of four contrasting drought-responsive chickpea genotypes: ICC 4958, JG 11, and JG 11+ (drought-tolerant), and ICC 1882 (drought-sensitive) under control and drought stress conditions. Integration of transcriptomics and proteomics data identified enriched hub proteins encoding isoflavone 4′-O-methyltransferase, UDP-d-glucose/UDP-d-galactose 4-epimerase, and delta-1-pyrroline-5-carboxylate synthetase. These proteins highlighted the involvement of pathways such as antibiotic biosynthesis, galactose metabolism, and isoflavonoid biosynthesis in activating drought stress response mechanisms. Subsequently, the integration of metabolomics data identified six metabolites (fructose, galactose, glucose, myoinositol, galactinol, and raffinose) that showed a significant correlation with galactose metabolism. Integration of root-omics data also revealed some key candidate genes underlying the drought-responsive “QTL-hotspot” region. These results provided key insights into complex molecular mechanisms underlying drought stress response in chickpea.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | chickpea, drought stress, Integrated multi-omics analysis |
| Subjects: | Mandate crops > Chickpea Others > Drought |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 22 Jan 2024 06:22 |
| Last Modified: | 22 Jan 2024 06:24 |
| URI: | http://oar.icrisat.org/id/eprint/12390 |
| Official URL: | https://acsess.onlinelibrary.wiley.com/doi/10.1002... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | Rajeev K. Varshney is thankful to Bill&Melinda Gates Foundation (OPP1114827) for supporting Tropical Legumes III project, and Science & Engineering Research Board (SERB) of Department of Science & Technology (DST) Government of India for providing the J C Bose National Fellowship (SB/S9/Z-13/2019). Himabindu Kudapa is thankful to the SERB of DST, Government of India, for providing SERB Women Excellence Award (SB/WEA-01/2017). Rutwik Barmukh acknowledges funding support from the Council of Scientific and Industrial Research (CSIR), Government of India, for the award of a research fellowship. |
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