Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance

Xu, K and Zhao, Y and Zhao, Yan and Feng, C and Zhang, Y and Wang, F and Li, X and Gao, H and Liu, W and Jing, Y and Saxena, R K and Feng, X and Zhou, Y and Li, H (2022) Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance. Frontiers in Plant Science (TSI), 13. pp. 1-13. ISSN 1664-462X

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Abstract

The F-box gene family is one of the largest gene families in plants. These genes regulate plant growth and development, as well as biotic and abiotic stress responses, and they have been extensively researched. Drought stress is one of the major factors limiting the yield and quality of soybean. In this study, bioinformatics analysis of the soybean F-box gene family was performed, and the role of soybean F-box-like gene GmFBL144 in drought stress adaptation was characterized. We identified 507 F-box genes in the soybean genome database, which were classified into 11 subfamilies. The expression profiles showed that GmFBL144 was highly expressed in plant roots. Overexpression of GmFBL144 increased the sensitivity of transgenic Arabidopsis to drought stress. Under drought stress, the hydrogen peroxide (H2O2) and malonaldehyde (MDA) contents of transgenic Arabidopsis were higher than those of the wild type (WT) and empty vector control, and the chlorophyll content was lower than that of the control. Y2H and bimolecular fluorescence complementation (BiFC) assays showed that GmFBL144 can interact with GmsHSP. Furthermore, our results showed that GmFBL144 can form SCFFBL144 (E3 ubiquitin ligase) with GmSkp1 and GmCullin1. Altogether, these results indicate that the soybean F-box-like protein GmFBL144 may negatively regulate plant drought stress tolerance by interacting with sHSP. These findings provide a basis for molecular genetics and breeding of soybean.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	soybean, F-box protein, drought stress, Skp1-Cullin1-F-box (SCF) complex, segmental duplication, protein interaction
Subjects:	Others > Drought Tolerance Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	17 Jan 2024 04:25
Last Modified:	17 Jan 2024 04:25
URI:	http://oar.icrisat.org/id/eprint/12370
Official URL:	https://www.frontiersin.org/articles/10.3389/fpls....
Projects:	UNSPECIFIED
Funders:	National Natural Science Foundation of China, Natural Science Foundation of Science Technology Department of Jilin Province, Hainan Provincial Natural Science Foundation of China, Scientific Research Foundation of Hainan University Program, Hainan Yazhou Bay Seed Laboratory
Acknowledgement:	We thank Bin Liu (Institute of Crop Science, Chinese Academy of Agricultural Sciences) for supplying the plasmid of BiFC. We also thank of Rong Huang for spiritual support.
Links:	Google Scholar

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