Identification of superior haplotypes in a diverse natural population for breeding desirable plant height in soybean

Bhat, J A and Karikari, B and Adeboye, K A and Ganie, S A and Barmukh, R and Hu, D and Varshney, R K and Yu, D (2022) Identification of superior haplotypes in a diverse natural population for breeding desirable plant height in soybean. Theoretical and Applied Genetics, 135. pp. 2407-2422. ISSN 1432-2242

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Abstract

Key message Plant height of soybean is associated with a haplotype block on chromosome 19, which classified 211 soybean accessions into five distinct groups showing significant differences for the target trait. Genetic variation is pivotal for crop improvement. Natural populations are precious genetic resources. However, efficient strategies for the targeted utilization of these resources for quantitative traits, such as plant height (PH), are scarce. Being an important agronomic trait associated with soybean yield and quality, it is imperative to unravel the genetic mechanisms underlying PH in soybean. Here, a genome-wide association study (GWAS) was performed to identify single nucleotide polymorphisms (SNPs) significantly associated with PH in a natural population of 211 cultivated soybeans, which was genotyped with NJAU 355 K Soy SNP Array and evaluated across six environments. A total of 128 SNPs distributed across 17 chromosomes were found to be significantly associated with PH across six environments and a combined environment. Three significant SNPs were consistently identified in at least three environments on Chr.02 (AX-93958260), Chr.17 (AX-94154834), and Chr.19 (AX-93897200). Genomic regions of ~ 130 kb flanking these three consistent SNPs were considered as stable QTLs, which included 169 genes. Of these, 22 genes (including Dt1) were prioritized and defined as putative candidates controlling PH. The genomic region flanking 12 most significant SNPs was in strong linkage disequilibrium (LD). These SNPs formed a single haplotype block containing five haplotypes for PH, namely Hap-A, Hap-B, Hap-C, Hap-D, and Hap-E. Deployment of such superior haplotypes in breeding programs will enable development of improved soybean varieties with desirable plant height.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Plant height, haplotype block, chromosome 19, soybean, Genetic variation
Subjects:	Others > Genetics and Genomics Others > Plant Growth
Depositing User:	Mr Nagaraju T
Date Deposited:	16 Jan 2024 08:20
Last Modified:	16 Jan 2024 08:20
URI:	http://oar.icrisat.org/id/eprint/12365
Official URL:	https://link.springer.com/article/10.1007/s00122-0...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Open Access funding enabled and organized by CAUL and its Member Institutions. This work was supported in part by National Natural Science Foundation of China (No. 32090065), Ministry of Science and Technology (No. 2017YFE0111000), and Horizon 2020 of European Union (EUCLEG/ No. 727312).
Links:	Google Scholar

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