Bhattacharya, J and Reddy, D S and Prasad, K and Nitnavare, R B and Bhatnagar-Mathur, P and Reddy, P S (2023) Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum. Gene, 868. pp. 1-7. ISSN 0378-1119
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Abstract
Orf147, a cytotoxic peptide, has been found to cause cytoplasmic male sterility (CMS) in Cajanus cajanifolius (pigeonpea). In our study, Orf147 was introduced into self-pollinating Cicer arietinum (chickpea) using Agrobacterium-mediated transformation for induction of CMS. The stable integration and expression of the transgene has been assessed through PCR and qRT-PCR analysis. In addition, phenotypic sterility analysis has been performed, considering developmental parameters like flower development, pod formation and flower drop. Transgene inheritance analysis demonstrates that out of the five PCR positive events in the T0 generation, two events have segregated according to the Mendelian segregation ratio (3:1) in the T2 generation. Further, pollen viability test using microscopic analysis confirms the induction of partial CMS in transgenic chickpea. The study holds significant value regarding the heterosis of self-pollinating legumes like chickpea. As a part of the prospect, exploring inducible promoters of species-specific or related legumes would be the next step to developing a two-line hybrid system.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC) |
| Uncontrolled Keywords: | Cytoplasmic male sterility, CMS, Heterosis, Transgenic chickpea, Orf147 |
| Subjects: | Others > Genetic Engineering Mandate crops > Chickpea Mandate crops > Pigeonpea |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 20 Nov 2023 03:18 |
| Last Modified: | 20 Nov 2023 03:18 |
| URI: | http://oar.icrisat.org/id/eprint/12305 |
| Official URL: | https://www.sciencedirect.com/science/article/abs/... |
| Projects: | UNSPECIFIED |
| Funders: | CGIAR Research Program on Grain Legumes & Dryland Cereals (CRP-GLDC) |
| Acknowledgement: | JB acknowledges DST INSPIRE fellowship received from Department of Science and Technology (DST/INSPIRE Fellowship/IF160547), Government of India. JB acknowledges the efforts of Mr N Kedarinath and Mr Vijay Tammali during the Agrobacterium-mediated transformation of chickpea and generation of transgenic lines. JB thanks Dr Mamta Sharma for providing the microscope facility for successful examination of pollen. |
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