Achola, E and Wasswa, P and Fonceka, D and Clevenger, J P and Bajaj, P and Ozias‑Akins, P and Rami, J and Deom, C M and Hoisington, D A and Edema, R and Odeny, D A and Okello, D K (2023) Genome‑wide association studies reveal novel loci for resistance to groundnut rosette disease in the African core groundnut collection. Theoretical and Applied Genetics, 136. pp. 1-20. ISSN 1432-2242
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Abstract
Groundnut is cultivated in several African countries where it is a major source of food, feed and income. One of the major constraints to groundnut production in Africa is groundnut rosette disease (GRD), which is caused by a complex of three agents: groundnut rosette assistor luteovirus, groundnut rosette umbravirus and its satellite RNA. Despite several years of breeding for GRD resistance, the genetics of the disease is not fully understood. The objective of the current study was to use the African core collection to establish the level of genetic variation in their response to GRD, and to map genomic regions responsible for the observed resistance. The African groundnut core genotypes were screened across two GRD hotspot locations in Uganda (Nakabango and Serere) for 3 seasons. The Area Under Disease Progress Curve combined with 7523 high quality SNPs were analyzed to establish marker-trait associations (MTAs). Genome-Wide Association Studies based on Enriched Compressed Mixed Linear Model detected 32 MTAs at Nakabango: 21 on chromosome A04, 10 on B04 and 1 on B08. Two of the significant markers were localised on the exons of a putative TIR-NBS-LRR disease resistance gene on chromosome A04. Our results suggest the likely involvement of major genes in the resistance to GRD but will need to be further validated with more comprehensive phenotypic and genotypic datasets. The markers identified in the current study will be developed into routine assays and validated for future genomics-assisted selection for GRD resistance in groundnut.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Genome‑wide association, novel loci, groundnut, rosette disease, Africa |
| Subjects: | Mandate crops > Groundnut Others > Plant Pathology Others > Africa |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 15 Nov 2023 04:39 |
| Last Modified: | 15 Nov 2023 04:39 |
| URI: | http://oar.icrisat.org/id/eprint/12299 |
| Official URL: | https://link.springer.com/article/10.1007/s00122-0... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | We acknowledge Essohouna Modom Banla, Dramane Sako, Issa Faye, Amade Muitia, Justus Chintu, Lutangu Makweti, Richard Oteng-Frimpong, James Asibuo and Adama Mamadou Coulibaly for contributing germplasm to the African core collection. We are also grateful to Erick Owuor Mikwa and Millicent Avosa for data and laboratory support, respectively. |
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