Genetic variation in CaTIFY4b contributes to drought adaptation in chickpea

Barmukh, R and Roorkiwal, M and Garg, V and Khan, A W and German, L and Jaganathan, D and Chitikineni, A and Kholova, J and Kudapa, H and Samineni, S and Sivasakthi, K and Kale, S M and Gaur, P M and Sagurthi, S R and Benitez-Alfonso, Y and Varshney, R K (2022) Genetic variation in CaTIFY4b contributes to drought adaptation in chickpea. Plant Biotechnology Journal, 20. pp. 1701-1715. ISSN 1467-7652

PDF - Published Version Available under License ["licenses_description_cc_attribution" not defined]. Download (1MB)

Abstract

Chickpea production is vulnerable to drought stress. Identifying the genetic components underlying drought adaptation is crucial for enhancing chickpea productivity. Here, we present the fine mapping and characterization of ‘QTL-hotspot’, a genomic region controlling chickpea growth with positive consequences on crop production under drought. We report that a non-synonymous substitution in the transcription factor CaTIFY4b regulates seed weight and organ size in chickpea. Ectopic expression of CaTIFY4b in Medicago truncatula enhances root growth under water deficit. Our results suggest that allelic variation in ‘QTL-hotspot’ improves pre-anthesis water use, transpiration efficiency, root architecture and canopy development, enabling high-yield performance under terminal drought conditions. Gene expression analysis indicated that CaTIFY4b may regulate organ size under water deficit by modulating the expression of GRF-INTERACTING FACTOR1 (GIF1), a transcriptional co-activator of Growth-Regulating Factors. Taken together, our study offers new insights into the role of CaTIFY4b and on diverse physiological and molecular mechanisms underpinning chickpea growth and production under specific drought scenarios.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	legumes, terminal drought, seed weight, root system architecture, transpiration efficiency, vigour
Subjects:	Mandate crops > Chickpea Others > Genetics and Genomics Others > Legume Crops
Depositing User:	Mr Nagaraju T
Date Deposited:	10 Nov 2023 11:10
Last Modified:	10 Nov 2023 11:11
URI:	http://oar.icrisat.org/id/eprint/12291
Official URL:	https://onlinelibrary.wiley.com/doi/full/10.1111/p...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	R.K.V. is grateful to Bill & Melinda Gates Foundation, USA, and Department of Biotechnology, Ministry of Science & Technology, Government of India, for supporting this research in parts. Thanks to Science & Engineering Research Board (SERB) of Department of Science & Technology (DST), Government of India, for providing the J C Bose National Fellowship (SB/S9/Z-13/2019) to R.K.V. R.B. acknowledges the funding support from Council of Scientific and Industrial Research (CSIR), Government of India, for the award of a research fellowship. The funding support from the Biotechnology and Biological Sciences Research Council (BBSRC) as a part of a UK-India Partnering Award for Plant Science for Food Security and Nutrition (Research grant number BB/R021171/1), is acknowledged for facilitating the exchange visit of R.B. to the University of Leeds. Work at Y.B.-A. lab is supported by UKRI Future Leader Fellowship (MR/T04263X/1), the Leverhulme Trust Grant RPG-2016-136, and the University of Leeds Gosden Studentship supporting L.G. Thanks are due to all colleagues and collaborators from ICRISAT and other organizations including Junichi Kashiwagi, L Krishnamurthy, Mahendar Thudi, Spurthi Nayak, Alice Kujur, Vikas Singh, Chellapilla Bharadwaj, NP Singh, Sushil K Chaturvedi, Roberto Tuberosa, Yusaku Uga, Kadambot Siddique, Timothy Colmer, Tim Sutton, Asnake Fikre, Paul Kimurto for their help and support in various ways to the "QTLhotspot" mapping and cloning project.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item