The location of iron and zinc in grain of conventional and biofortified lines of sorghum

Gaddameedi, A and Sheraz, S and Kumar, A and Li, K and Pellny, T and Gupta, R and Wan, Y and Moore, K L and Shewry, P R (2022) The location of iron and zinc in grain of conventional and biofortified lines of sorghum. Journal of Cereal Science (TSI), 107. pp. 1-7. ISSN 0733-5210

PDF - Published Version Available under License Creative Commons Attribution. Download (9MB)

Abstract

Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of 56Fe+ and 64Zn+ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31P16O+ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56Fe+ and 64Zn+ were also observed in the protein matrix of these cells

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Sorghum grain, Biofortification, NanoSIMS, Localisation, Iron, Zinc
Subjects:	Others > Biofortification Mandate crops > Sorghum Others > Food and Nutrition
Depositing User:	Mr Nagaraju T
Date Deposited:	10 Nov 2023 03:40
Last Modified:	10 Nov 2023 03:40
URI:	http://oar.icrisat.org/id/eprint/12288
Official URL:	https://www.sciencedirect.com/science/article/pii/...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Rothamsted Research receives strategic funding from the Biotechnology and Biological Sciences Research Council (BBSRC) and the work at Rothamsted Research forms part of the Designing Future Wheat strategic programme (BB/P016855/1). The work was also supported by BBSRC Newton Fund Grant BB/N013360/1 Indo-UK Centre for the improvement of Nitrogen use Efficiency in Wheat (INEW), the Cambridge India Network for Translational Research in Nitrogen (CINTRIN) supported by the Department of Biotechnology (Grant BT-IN-UK-VNC-42-RG-2015-16), Government of India and the BBSRC (BB/P027970/1) and BBSRC grants BB/P019072/1 and BB/P019358/1 CROPNUT: increasing iron in cereals. The NanoSIMS was funded by UK Research Partnership Investment Funding (UKRPIF) Manchester RPIF Round 2. This work was supported by the Henry Royce Institute for Advanced Materials, funded through EPSRC grants EP/R00661X/1, EP/S019367/1, EP/P025021/1 and EP/P025498/1.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item